苎麻脱胶共生菌群RAMCD407中两株优势菌的分离、鉴定和脱胶能力分析

为了从苎麻脱胶共生菌群RAMCD407中分离高效脱胶菌种,利用苎麻发酵物培养基分离筛选高效脱胶菌株;形态、生化结合16S rDNA分子方法鉴定菌株;水解圈法结合酶活力测定验证入选菌株产酶能力;苎麻脱胶试验核实入选菌株脱胶能力;分离出K30和L11两个菌株。K30和L11都能在果胶和木聚糖平板上形成水解圈,K30的果胶酶和木聚糖酶活力分别为238.47 U/mL和98.36 U/mL,L11的果胶酶和木聚糖酶活力分别为170.79 U/mL和64.11 U/mL;K30和L11进行苎麻生物脱胶时,能使纤维残胶率由32.3%下降为12.43%和15.70%,纤维强力均超过5.5 cN/dtex。V...

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Bibliographic Details
Published in中国农业科技导报 Vol. 15; no. 4; pp. 144 - 150
Main Author 余苗 陈洪高 王超群 施薇
Format Journal Article
LanguageChinese
Published 武汉纺织大学纺织印染清洁生产教育部工程研究中心,武汉,430073 2013
Subjects
共生菌群
分离
芽孢杆菌
苎麻脱胶
鉴定
ramie degumming
identification
鉴定
bacteria consortium
芽孢杆菌
isolation
苎麻脱胶
共生菌群
Bacillaceae
分离
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ISSN1008-0864
DOI10.3969/j.issn.1008-0864.2013.04.22

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Summary:为了从苎麻脱胶共生菌群RAMCD407中分离高效脱胶菌种,利用苎麻发酵物培养基分离筛选高效脱胶菌株;形态、生化结合16S rDNA分子方法鉴定菌株;水解圈法结合酶活力测定验证入选菌株产酶能力;苎麻脱胶试验核实入选菌株脱胶能力;分离出K30和L11两个菌株。K30和L11都能在果胶和木聚糖平板上形成水解圈,K30的果胶酶和木聚糖酶活力分别为238.47 U/mL和98.36 U/mL,L11的果胶酶和木聚糖酶活力分别为170.79 U/mL和64.11 U/mL;K30和L11进行苎麻生物脱胶时,能使纤维残胶率由32.3%下降为12.43%和15.70%,纤维强力均超过5.5 cN/dtex。VITEK系统生化鉴定和16S rDNA序列分析都一致表明,K30与枯草芽孢杆菌Bacillus subtilis相似性高达99%,L11与蜡样芽胞杆菌Bacillus cereus相似性达到99%。
Bibliography:ramie degumming; bacteria consortium; Bacillaceae; isolation; identification
In order to isolate high efficient degumming strains from a ramie retting bacterial consortium RAMCD407, a specific solid medium mainly consisted of ramie gum materials from ramie retting liquids by RAMCIM07 was utilized for strain screening and isolating. Biochemical methods combined with 16S rRNA gene sequencing and morphological discrimination were chosen for strain identification. Enzyme activity analysis and practical degumming experiments were adopted to test the potential of selected strains in ramie degumming industries. Two strains designated K30 and L11 were obtained after purification and screening. Both 2 strains can form hydrolysis halos either on the plate containing pectin or xylan. The pectinase activities were 238.47 U/mL for [（30 and 170.79 U/mL for Lll. The xylanase activities were 98.36 U/mL for K30 and 64.11 U/mL for L11. The residual gum contents of ramie fibers were reduced from 32.3% to 12.43% and 15.70% , resp
ISSN:1008-0864
DOI:10.3969/j.issn.1008-0864.2013.04.22