Normal acute and chronic inflammatory responses in sphingosine kinase 1 knockout mice

• Published in: FEBS Letters Vol. 580; no. 19; pp. 4607 - 4612
• Main Authors: Michaud, Jason, Kohno, Masataka, Proia, Richard L., Hla, Timothy
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 21.08.2006; Wiley
• Subjects: Acute Disease; Animals; Arthritis; Base Sequence; Chronic Disease; CIA; Collagen-induced arthritis; DNA Primers; Inflammation; Inflammation - metabolism; Male; Mice; Mice, Knockout; N-Formylmethionine Leucyl-Phenylalanine; N-Formylmethionine Leucyl-Phenylalanine - metabolism; NADPH Oxidases; NADPH Oxidases - metabolism; Neutrophils; Neutrophils - enzymology; Phosphotransferases (Alcohol Group Acceptor); Phosphotransferases (Alcohol Group Acceptor) - genetics; Phosphotransferases (Alcohol Group Acceptor) - metabolism; S1P; Signal Transduction; Sphingolipids; Sphingosine kinase; Sphingosine kinase 1; Sphingosine-1-phosophate; Sphingosine-1-phosphate lyase; Sphingosine-1-phosphate phosphatase; Sphk1; SPL; Spp; Spp; Sphingosine kinase; S1P; Sphk1; CIA; Sphingolipids; Inflammation; Arthritis; SPL
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/j.febslet.2006.07.035

Sphingosine-1-phosophate, generated from the phosphorylation of sphingosine by sphingosine kinase enzymes, is suggested to function as an intracellular second messenger for inflammatory mediators, including formyl peptide, C5a, and Fc. More recently, a role for sphingosine kinases during inflammation has also been proposed. Here we show that sphingosine kinase 1 knockout mice exhibit normal inflammatory cell recruitment during thioglycollate-induced peritonitis and that sphingosine kinase 1-null neutrophils respond normally to formyl peptide. In the collagen-induced arthritis model of rheumatoid arthritis, sphingosine kinase 1 knockout mice developed arthritis with normal incidence and severity. Our findings show that sphingosine kinase 1 is dispensable for inflammatory responses and support the need for more extensive studies of sphingosine kinases in inflammation.