皮层扩散性抑制对蛛网膜下腔出血后皮层脑血流及细胞凋亡的影响

目的明确大鼠蛛网膜下腔出血（subarachnoidhemorrhage，SAH）后皮层扩散性抑制（corticalspreadingdepression，CSD）对皮层脑血流及细胞凋亡的影响。方法采用枕大池2次注血法建立大鼠SAH模型，利用皮层给以KCl的方法诱导CSD的发生，激光多普勒血流仪行皮层脑血流测定，TUNEL染色检测细胞凋亡。结果SAH组大鼠脑皮层血流较空白组和盐水组明显下降，其中5d组最低；皮层细胞凋亡水平明显升高，5d组最明显；CSD发生后SAH组脑血流下降更明显，凋亡更严重，空白组和盐水组变化不明显。结论SAH后CSD的发生使脑血流进一步下降，皮层细朐的凋亡更加严重，CSD...

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Published in西安交通大学学报（医学版） Vol. 34; no. 6; pp. 754 - 757
Main Author 郝璞珩 宋锦宁 陈虎 安吉洋 闰文涛 李宇 王军锋 张明
Format Journal Article
LanguageChinese
Published 西安医学院第一附属,医院神经外二科,陕西西安,710077%西安交通大学医学院第一附属医院神经外科,陕西西安,710061%第四军医大学唐都医院神经外科,陕西西安,710032%许昌市中心医院神经外科,河南许昌,461000 2013
西安交通大学医学院第一附属医院神经外科,陕西西安,710061
Subjects
凋亡
大鼠
皮层扩散性抑制
脑血流
蛛网膜下腔出血
皮层扩散性抑制
大鼠
凋亡
蛛网膜下腔出血
脑血流
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ISSN1671-8259
DOI10.7652/jdyxb201306011

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Summary:目的明确大鼠蛛网膜下腔出血（subarachnoidhemorrhage，SAH）后皮层扩散性抑制（corticalspreadingdepression，CSD）对皮层脑血流及细胞凋亡的影响。方法采用枕大池2次注血法建立大鼠SAH模型，利用皮层给以KCl的方法诱导CSD的发生，激光多普勒血流仪行皮层脑血流测定，TUNEL染色检测细胞凋亡。结果SAH组大鼠脑皮层血流较空白组和盐水组明显下降，其中5d组最低；皮层细胞凋亡水平明显升高，5d组最明显；CSD发生后SAH组脑血流下降更明显，凋亡更严重，空白组和盐水组变化不明显。结论SAH后CSD的发生使脑血流进一步下降，皮层细朐的凋亡更加严重，CSD可能是导致迟发性缺血性神经功能障碍的机制之一。
Bibliography:HAO Pu-heng1＇2 , SONG Jin-ning1 , CHEN Hu1 , AN Ji-yang1 YAN Wen-tao4 , LI Yu1 , WANG Jun-fengI , ZHANG MingI （1. Department of Neurosurgery, the First Affiliated Hospital, Medical School of Xi＇an Jiaotong University, Xi＇an 710061; 2. Department 2 of Neurosurgery, the First Affiliated Hospital, Xi＇an Medical College, Xi＇an 710077; 3. Department of Neurosurgery, Tangdu Hospital of Fourth Military Medical University, Xi＇an 710032; 4. Department of Neurosurgery, Xuehang Central Hospital, Xuchang 461000, China）
subarachnoid hemorrhage; cortical spreading depression; cerebral blood flow~ apoptosis
Objective To clarify the effect of cortical spreading depression （CSD） on cortical cerebral blood flow and apoptosis following subarachnoid hemorrhage （SAH） in rats. Methods SAH was induced in male Wistar rats by autologous blood injection into the cisterna magna and CSD was induced in rats with KC1. The cortical blood flow was measured by laser Doppler flowmeter and apoptosis were detected by TUNEL staining. Results In SA
ISSN:1671-8259
DOI:10.7652/jdyxb201306011