Twist表达增强乳腺癌MCF-7细胞的多药耐药性
目的探讨MCF7／Twist稳定表达细胞株的多药耐药性。方法MCF7细胞中转染Twist，G418筛选，建立稳定表达细胞株MCF－7／Twist，Westernblotting法检测Twist表达；观察增殖状态；MTT法测阿霉素、紫杉醇、长春新碱和羟喜树碱对细胞株增殖活性的影响，分析稳定转染细胞株对药物的敏感性；应用RT—PCR及Westernblotting方法，检测耐药相关糖蛋白P—gp和乳腺癌耐药蛋白BCRP转录水平和蛋白水平的变化。结果G418筛选获得稳定表达Twist的细胞株，对阿霉素、紫杉醇、长春新碱和羟喜树碱具有明显耐药性，耐药相关分子P—gP和BCRP转录和表达明显升高。结论T...
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| Published in | 西安交通大学学报（医学版） Vol. 34; no. 1; pp. 89 - 92 |
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| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
西北大学生命科学学院,人口与健康研究所,陕西西安 710069%西安市儿童医院病理科,陕西西安,710003
2013
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1671-8259 |
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| Abstract | 目的探讨MCF7／Twist稳定表达细胞株的多药耐药性。方法MCF7细胞中转染Twist，G418筛选，建立稳定表达细胞株MCF－7／Twist，Westernblotting法检测Twist表达；观察增殖状态；MTT法测阿霉素、紫杉醇、长春新碱和羟喜树碱对细胞株增殖活性的影响，分析稳定转染细胞株对药物的敏感性；应用RT—PCR及Westernblotting方法，检测耐药相关糖蛋白P—gp和乳腺癌耐药蛋白BCRP转录水平和蛋白水平的变化。结果G418筛选获得稳定表达Twist的细胞株，对阿霉素、紫杉醇、长春新碱和羟喜树碱具有明显耐药性，耐药相关分子P—gP和BCRP转录和表达明显升高。结论Twist表达增强乳腺癌细胞的多药耐药性，多药耐药性相关分子的转录和表达升高与耐药性增强现象一致。 |
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| AbstractList | 目的 探讨MCF-7/Twist稳定表达细胞株的多药耐药性.方法 MCF-7细胞中转染Twist,G418筛选,建立稳定表达细胞株MCF-7/Twist,Western blotting法检测Twist表达；观察增殖状态；MTT法测阿霉素、紫杉醇、长春新碱和羟喜树碱对细胞株增殖活性的影响,分析稳定转染细胞株对药物的敏感性；应用RT-PCR及Western blotting方法,检测耐药相关糖蛋白 P-gp和乳腺癌耐药蛋白BCRP转录水平和蛋白水平的变化.结果 G418筛选获得稳定表达Twist的细胞株,对阿霉素、紫杉醇、长春新碱和羟喜树碱具有明显耐药性,耐药相关分子P-gp和BCRP转录和表达明显升高.结论 Twist表达增强乳腺癌细胞的多药耐药性,多药耐药性相关分子的转录和表达升高与耐药性增强现象一致. 目的探讨MCF7／Twist稳定表达细胞株的多药耐药性。方法MCF7细胞中转染Twist，G418筛选，建立稳定表达细胞株MCF－7／Twist，Westernblotting法检测Twist表达；观察增殖状态；MTT法测阿霉素、紫杉醇、长春新碱和羟喜树碱对细胞株增殖活性的影响，分析稳定转染细胞株对药物的敏感性；应用RT—PCR及Westernblotting方法，检测耐药相关糖蛋白P—gp和乳腺癌耐药蛋白BCRP转录水平和蛋白水平的变化。结果G418筛选获得稳定表达Twist的细胞株，对阿霉素、紫杉醇、长春新碱和羟喜树碱具有明显耐药性，耐药相关分子P—gP和BCRP转录和表达明显升高。结论Twist表达增强乳腺癌细胞的多药耐药性，多药耐药性相关分子的转录和表达升高与耐药性增强现象一致。 |
| Author | 李军林 杨星 李美婷 赵欢 陈广生 高晓彩 |
| AuthorAffiliation | 西北大学生命科学学院,人口与健康研究所,陕西西安710069 西安市儿童医院病理科,陕西西安710003 |
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| Author_FL | YANG Xing ZHAO Huan GAO Xiao-cai LI Jun-lin LI Mei-ting CHEN Guang-sheng |
| Author_FL_xml | – sequence: 1 fullname: LI Jun-lin – sequence: 2 fullname: YANG Xing – sequence: 3 fullname: LI Mei-ting – sequence: 4 fullname: ZHAO Huan – sequence: 5 fullname: CHEN Guang-sheng – sequence: 6 fullname: GAO Xiao-cai |
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| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
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| DocumentTitleAlternate | Enhanced muitidrug resistance by Twist stable expression in MCF-7 cell line of human breast cancer |
| DocumentTitle_FL | Enhanced multidrug resistance by Twist stable expression in MCF-7 cell line of human breast cancer |
| EndPage | 92 |
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| Keywords | Twist 稳定转染 多药耐药 乳腺癌 MCF-7 |
| Language | Chinese |
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| Notes | LI Jun-lin , YANG King , LI Mei-ting , ZHAO Huan , CHEN Guang-sheng , GAO Xiao-cai （1. Institute of Population and Health, College of Life Science, Northwest University, Xi＇an 710069; 2. Department of Pathology, Xi＇an Children＇s Hospital, Xi＇an 710003, China） breast cancer ; multidrug resistance （MDR） ; MCF-7 ; Twist; stable transfection Objective To study multidrug resistance （MDR） enhanced by Twist stable expression in MCF-7 cell line of human breast cancer in vitro. Methods Twist gene was introduced into MCF-7 to establish a stable expression of the cell line MCF-7/Twist. The expression of Twist was detected with Western blotting. The cell proliferation and viability were observed under microscope and measured with MTT assay. Reverse transcription- polymerase chain reaction was used to test transcriptional level of genes involved in MDR. The relevant protein expression level of P-gp and breast cancer resistance protein （BCRP） was detected with Western blotting. Results The results showed low sensitivity of M |
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| PublicationTitle | 西安交通大学学报（医学版） |
| PublicationTitleAlternate | Journal of Xi‘an Jiaotong University：Medical Sciences |
| PublicationTitle_FL | Journal of Xi'an Jiaotong University(Medical Sciences) |
| PublicationYear | 2013 |
| Publisher | 西北大学生命科学学院,人口与健康研究所,陕西西安 710069%西安市儿童医院病理科,陕西西安,710003 |
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| Snippet | 目的探讨MCF7／Twist稳定表达细胞株的多药耐药性。方法MCF7细胞中转染Twist，G418筛选，建立稳定表达细胞株MCF－7／Twist，Westernblotting法检测Twist表达；观察增殖状... 目的 探讨MCF-7/Twist稳定表达细胞株的多药耐药性.方法 MCF-7细胞中转染Twist,G418筛选,建立稳定表达细胞株MCF-7/Twist,Western blotting法检测Twist表达；观察增殖状... |
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| SubjectTerms | MCF-7 Twist 乳腺癌 多药耐药 稳定转染 |
| Title | Twist表达增强乳腺癌MCF-7细胞的多药耐药性 |
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