Twist表达增强乳腺癌MCF-7细胞的多药耐药性

目的探讨MCF7／Twist稳定表达细胞株的多药耐药性。方法MCF7细胞中转染Twist，G418筛选，建立稳定表达细胞株MCF－7／Twist，Westernblotting法检测Twist表达；观察增殖状态；MTT法测阿霉素、紫杉醇、长春新碱和羟喜树碱对细胞株增殖活性的影响，分析稳定转染细胞株对药物的敏感性；应用RT—PCR及Westernblotting方法，检测耐药相关糖蛋白P—gp和乳腺癌耐药蛋白BCRP转录水平和蛋白水平的变化。结果G418筛选获得稳定表达Twist的细胞株，对阿霉素、紫杉醇、长春新碱和羟喜树碱具有明显耐药性，耐药相关分子P—gP和BCRP转录和表达明显升高。结论T...

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Published in西安交通大学学报（医学版） Vol. 34; no. 1; pp. 89 - 92
Main Author 李军林 杨星 李美婷 赵欢 陈广生 高晓彩
Format Journal Article
LanguageChinese
Published 西北大学生命科学学院,人口与健康研究所,陕西西安 710069%西安市儿童医院病理科,陕西西安,710003 2013
Subjects
MCF-7
Twist
乳腺癌
多药耐药
稳定转染
Twist
稳定转染
多药耐药
乳腺癌
MCF-7
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ISSN1671-8259

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Summary:目的探讨MCF7／Twist稳定表达细胞株的多药耐药性。方法MCF7细胞中转染Twist，G418筛选，建立稳定表达细胞株MCF－7／Twist，Westernblotting法检测Twist表达；观察增殖状态；MTT法测阿霉素、紫杉醇、长春新碱和羟喜树碱对细胞株增殖活性的影响，分析稳定转染细胞株对药物的敏感性；应用RT—PCR及Westernblotting方法，检测耐药相关糖蛋白P—gp和乳腺癌耐药蛋白BCRP转录水平和蛋白水平的变化。结果G418筛选获得稳定表达Twist的细胞株，对阿霉素、紫杉醇、长春新碱和羟喜树碱具有明显耐药性，耐药相关分子P—gP和BCRP转录和表达明显升高。结论Twist表达增强乳腺癌细胞的多药耐药性，多药耐药性相关分子的转录和表达升高与耐药性增强现象一致。
Bibliography:LI Jun-lin , YANG King , LI Mei-ting , ZHAO Huan , CHEN Guang-sheng , GAO Xiao-cai （1. Institute of Population and Health, College of Life Science, Northwest University, Xi＇an 710069; 2. Department of Pathology, Xi＇an Children＇s Hospital, Xi＇an 710003, China）
breast cancer ; multidrug resistance （MDR） ; MCF-7 ; Twist; stable transfection
Objective To study multidrug resistance （MDR） enhanced by Twist stable expression in MCF-7 cell line of human breast cancer in vitro. Methods Twist gene was introduced into MCF-7 to establish a stable expression of the cell line MCF-7/Twist. The expression of Twist was detected with Western blotting. The cell proliferation and viability were observed under microscope and measured with MTT assay. Reverse transcription- polymerase chain reaction was used to test transcriptional level of genes involved in MDR. The relevant protein expression level of P-gp and breast cancer resistance protein （BCRP） was detected with Western blotting. Results The results showed low sensitivity of M
ISSN:1671-8259