桑叶γ-氨基丁酸提取工艺的响应面优化

利用逐因子试验和Box-Behnken试验,以水为溶剂对桑叶γ-氨基丁酸（GABA）的萃取工艺进行了条件优化研究。结果表明,最佳提取条件为,桑叶粉∶水=1（g）∶30（m L）,提取温度50℃,提取时间2.13h,此工艺条件下提取率为0.464%,其中影响因素贡献率为提取时间﹥料液比﹥提取温度。...

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Bibliographic Details
Published in广东农业科学 Vol. 43; no. 7; pp. 120 - 125
Main Author 钟杨生 陈恒文 林碧敏 林健荣 陈芳艳
Format Journal Article
LanguageChinese
Published 华南农业大学动物科学学院,广东广州,510642%华南农业大学公共基础课实验教学中心,广东广州,510642 2016
Subjects
Box-Behnken试验
γ-氨基丁酸
提取
桑叶
Box-Behnken试验
桑叶
提取
γ-氨基丁酸
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ISSN1004-874X
DOI10.16768/j.issn.1004-874X.2016.07.019

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Summary:利用逐因子试验和Box-Behnken试验,以水为溶剂对桑叶γ-氨基丁酸（GABA）的萃取工艺进行了条件优化研究。结果表明,最佳提取条件为,桑叶粉∶水=1（g）∶30（m L）,提取温度50℃,提取时间2.13h,此工艺条件下提取率为0.464%,其中影响因素贡献率为提取时间﹥料液比﹥提取温度。
Bibliography:44-1267/S
mulberry leaf; γ-aminobutyric acid（GABA）; extraction; Box-Behnken test
By seriatim-factorial experiment and Box-Behnken test,the extraction conditions of γ-aminobutyric acid（GABA） from mulberry leaf with water were optimized. The results showed that the extraction rate was 0.464% under the following modification conditions： the ratio of mulberry leaf powder to water was 1（g）∶30（m L） and treated at 50℃ for 2.13 h. The order of the factors influencing the result was extraction time ﹥ ratio of mulberry leaf powder to water ﹥ temperature.
ZHONG Yang-sheng;CHEN Heng-wen;LIN Bi-min;LIN Jian-rong;CHEN Fang-yan （ 1. College of Animal Science, South China Agricultural University, Guangzhou 510642, China ; 2.Public Basic Courses Experimental Teaching Center, South China Agricultural University, Guangzhou 510642, China ）
ISSN:1004-874X
DOI:10.16768/j.issn.1004-874X.2016.07.019