云南松主分布区天然群体的遗传多样性及保护单元的构建

以20个云南松天然群体为对象,SSR分析表明云南松遗传变异主要存在于群体内,各群体间的遗传多样性水平差异不显著。基于SSR分子标记遗传多样性分析,根据Nei’s遗传相似度,采用逐步聚类优先取样法,分别对初始群体、遗传多样性保护单元和剩余群体进行比较、t检验,以此评价遗传多样性保护单元的代表性。结果表明,利用该方法抽样的群体能很好地代表初始群体的遗传多样性,按30%的群体抽样,等位基因数、有效等位基因数、Shannon’s信息指数和期望杂合度的保留率分别达到初始群体的98.03%、105.36%、103.99%和105.56%。保护单元的遗传多样性均高于剩余群体,抽样群体组成的遗传多样性保护单元...

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Bibliographic Details
Published in林业科学研究 Vol. 28; no. 6; pp. 883 - 891
Main Author 许玉兰 蔡年辉 徐杨 何承忠 王大玮 陈诗 段安安
Format Journal Article
LanguageChinese
Published 西南林业大学,国家林业局西南地区生物多样性保育重点实验室,云南昆明650224 2015
西南林业大学,西南山地森林保育与利用省部共建教育部重点实验室,云南昆明650224%西南林业大学,西南山地森林保育与利用省部共建教育部重点实验室,云南昆明650224%西南林业大学,云南省高校林木遗传改良与繁育重点实验室,云南昆明650224
西南林业大学,云南省高校林木遗传改良与繁育重点实验室,云南昆明650224
Subjects
云南松
保护单元
天然群体
种质资源
遗传多样性
genetic diversity
germplasm resources
conservation unit
遗传多样性
种质资源
云南松
natural populations
Pinus yunnanensis
天然群体
保护单元
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ISSN1001-1498

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Summary:以20个云南松天然群体为对象,SSR分析表明云南松遗传变异主要存在于群体内,各群体间的遗传多样性水平差异不显著。基于SSR分子标记遗传多样性分析,根据Nei’s遗传相似度,采用逐步聚类优先取样法,分别对初始群体、遗传多样性保护单元和剩余群体进行比较、t检验,以此评价遗传多样性保护单元的代表性。结果表明,利用该方法抽样的群体能很好地代表初始群体的遗传多样性,按30%的群体抽样,等位基因数、有效等位基因数、Shannon’s信息指数和期望杂合度的保留率分别达到初始群体的98.03%、105.36%、103.99%和105.56%。保护单元的遗传多样性均高于剩余群体,抽样群体组成的遗传多样性保护单元的效果较好。
Bibliography:The intra-specific variation of 20 Pinus yunnanensis Franch. natural populations collected from the main distribution areas was analyzed. The results revealed higher genetic variation within populations than among popula- tions based on SSR data and the genetic variation existed mainly within populations. There were not significantly genetic differences among populations. Based on SSR data and Nei＇ s genetic similarity and the level of genetic.＂ diversity, the conservation units of P. yunnanensis were analyzed by using stepwise clustering and preferred sampling. The representativeness of sampling were evaluated by T-test among primary populations, conservation units and re- mainder populations. The results showed this method was effective to establish the conservation units. The CU6 composed of six P. yunnanensis natural populations had the 30% germplasm samples of primary populations. The retention ratio of number of alleles, effective number of alleles, Shannon＇ s information index and expected heterozy- go
ISSN:1001-1498