低温胁迫对甘蔗幼苗根系线粒体生理生化作用的影响

【目的】探讨低温胁迫对甘蔗根系线粒体生理生化作用的影响,为甘蔗抗寒育种提供参考依据。【方法】采用水培系统培养甘蔗幼苗,并于人工气候室在4℃下进行低温处理,以25℃培养为对照。采集不同处理天数甘蔗根系样品分离提取线粒体,进行超微结构观察及生理生化分析。【结果】低温胁迫导致甘蔗根系线粒体结构模糊、膜破裂或消失,嵴断裂、减少,线粒体解体;抗寒性强的GT28受损伤程度低于抗寒性弱的ROC22;甘蔗根系线粒体超氧阴离子自由基(O2^-·)含量和抗氧化防护系统相关酶POD、CAT活性升高,且GT28的POD、CAT活性高于ROC22的对应值,而O2^-·含量低于ROC22的对应值;与对照相比,低温胁迫后G...

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Published in南方农业学报 Vol. 46; no. 8; pp. 1385 - 1390
Main Author 陈荣发 刘文娟 张保青 杨丽涛 李杨瑞
Format Journal Article
LanguageChinese
Published 亚热带农业生物资源保护与利用国家重点实验室,南宁530005 2015
广西农业科学院甘蔗研究所/中国农业科学院甘蔗研究中心/广西甘蔗遗传改良重点实验室,南宁530007
广西大学农学院,南宁530005
亚热带农业生物资源保护与利用国家重点实验室,南宁530005%广西大学农学院,南宁530005
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ISSN2095-1191
DOI10.3969/j:issn.2095-1191.2015.08.1385

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Summary:【目的】探讨低温胁迫对甘蔗根系线粒体生理生化作用的影响,为甘蔗抗寒育种提供参考依据。【方法】采用水培系统培养甘蔗幼苗,并于人工气候室在4℃下进行低温处理,以25℃培养为对照。采集不同处理天数甘蔗根系样品分离提取线粒体,进行超微结构观察及生理生化分析。【结果】低温胁迫导致甘蔗根系线粒体结构模糊、膜破裂或消失,嵴断裂、减少,线粒体解体;抗寒性强的GT28受损伤程度低于抗寒性弱的ROC22;甘蔗根系线粒体超氧阴离子自由基(O2^-·)含量和抗氧化防护系统相关酶POD、CAT活性升高,且GT28的POD、CAT活性高于ROC22的对应值,而O2^-·含量低于ROC22的对应值;与对照相比,低温胁迫后GT28的SOD活性明显升高,而ROC22的明显降低。【结论】甘蔗根系线粒体结构的损伤程度与品种抗寒性呈负相关,抗寒性较弱的ROC22比GT28更易发生膜脂过氧化,使线粒体性能发生早衰,加速细胞死亡。
Bibliography:45-1381/S
sugarcane; roots; low-temperature stress; mitochondria; physiological and biochemical characteristics
Objective】The present study was conducted to investigate effects of low-temperature stress on physiological and biochemical functions of mitochondria in sugarcane root, in order to provide references for breeding cold-resistant varity.【Method】The hydroponic culture system was appiled to cultivate sugarcane seedlings, and these seedlings were exposed to low-temperature stress of 4 ℃ in an artificial climate chamber, using seedlings cultivated at 25 ℃ as control. Then their roots were collected at different times, and the mitochondrias were isolated from roots to observe its ultrastructure and analyse its physiological and biochemical function under low temperature stress. 【Result 】The results showed that under low temperature stress, the root mitochondrial structure was getting obscure, the membrane was broken or disappeared, the cristae were destroyed and reduced, and the mitochondria began to disinte
ISSN:2095-1191
DOI:10.3969/j:issn.2095-1191.2015.08.1385