环境因子对轻腌大黄鱼中溶藻弧菌生长/非生长界面的影响

为建立生长/非生长界面模型来预测溶藻弧菌在环境因子交互作用下的生长概率,探究环境因子对溶藻弧菌的生长的交互作用,该文选取轻腌大黄鱼室温25℃贮藏货架期终点分离的溶藻弧菌作为研究对象,研究25℃下p H值、水分活度（water activity,aw）和盐分对其生长概率的交互影响。使用Gompertz模型对其生长情况进行拟合,比较其生长动力学参数。用简单Logistic方程、二阶线性Logistic回归方程拟合及概率神经网络算法（probabilistic neural network,PNN）建立溶藻弧菌生长/非生长界面模型,使用正确率、假阳性率对其拟合优度进行比较。结果表明：二阶线性Logi...

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Bibliographic Details
Published in农业工程学报 Vol. 34; no. 3; pp. 292 - 299
Main Author 郭全友;朱彦祺;姜朝军;李保国
Format Journal Article
LanguageChinese
Published 上海理工大学医疗器械与食品学院,上海 200093%上海理工大学医疗器械与食品学院,上海,200093 2018
中国水产科学研究院东海水产研究所,上海,200090%中国水产科学研究院东海水产研究所,上海 200090
Subjects
模型
溶藻弧菌
环境影响
生长动力学
菌
轻腌大黄鱼
鱼
轻腌大黄鱼
models
bacteria
vibrio alginolyticus
模型
environmental impact
growth kinetics
菌
pseudosciaena crocea
fish
生长动力学
鱼
溶藻弧菌
环境影响
Online AccessGet full text
ISSN1002-6819
DOI10.11975/j.issn.1002-6819.2018.03.039

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Summary:为建立生长/非生长界面模型来预测溶藻弧菌在环境因子交互作用下的生长概率,探究环境因子对溶藻弧菌的生长的交互作用,该文选取轻腌大黄鱼室温25℃贮藏货架期终点分离的溶藻弧菌作为研究对象,研究25℃下p H值、水分活度（water activity,aw）和盐分对其生长概率的交互影响。使用Gompertz模型对其生长情况进行拟合,比较其生长动力学参数。用简单Logistic方程、二阶线性Logistic回归方程拟合及概率神经网络算法（probabilistic neural network,PNN）建立溶藻弧菌生长/非生长界面模型,使用正确率、假阳性率对其拟合优度进行比较。结果表明：二阶线性Logistic回归方程拟合效果更优,验证集的正确率为90.9%,PNN验证集正确率为90.0%。随着盐分的增大,生长/非生长的界限明显向低水分活度、低p H值方向移动;在相同盐分条件范围内,高水分活度且p H值较高条件下,比生长速率较高,延滞期也相应较短;随着盐分的增长,0.91与0.90低水分活度条件下溶藻弧菌也开始缓慢增长,但存在较长时间的延滞期,高盐分对溶藻弧菌有生长促进作用。研究表明：PNN可在工业生产中对溶藻弧菌的生长和非生长快速预测分类,通过二阶线性Logistic可评估p H值、aw和盐分许用范围内水产品的安全性。通过构建溶藻弧菌概率模型和动力学模型,可为改进贮藏条件和产品配方、确保轻腌大黄鱼质量安全提供支持。
Bibliography:11-2047/S
fish; bacteria; models; growth kinetics; environmental impact; vibrio alginolyticus; pseudosciaena crocea
Guo Quanyou1, Zhu Yanqi1,2, Jiang Chaojun1, Li Baoguo2 （1. East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China; 2. School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China）
The aim of the study was to develop a growth/no-growth interface model to predict the growth probability of Vibrio alginolyticus associated with lightly salted Pseudosciaena crocea under 3 environmental factors, and to explore the inhibitory effect of environmental factors on the growth kinetics of target micro-organism. The effects of p H value, water activity（aw） and NaC l content on the growth probability of the Vibrio alginolytica were studied at ambient temperature（25 ℃）. At present, mainly the ＂fence technology＂ is used to change the growth environment of microorganisms by changing the water activity, sa
ISSN:1002-6819
DOI:10.11975/j.issn.1002-6819.2018.03.039