草莓轻型黄边病毒检测研究

本文对草莓轻型黄边病毒（Strawberry mild yellow edge virus，SMYEV）的2种分子检测技术进行了比较研究。结果表明，多重PCR技术以草莓actin为内参基因，完全排除在实验中杂质与操作的影响。Real time PCR技术具有更高的灵敏度和可靠性，可以定量检测出样本中病毒粒子的含量。结果表明，Real time PCR的灵敏度是普通PCR的100倍以上，而多重PCR的检测成本更经济。所以Real time PCR技术将用于引进品种的检疫，而多重PCR技术将用于大规模的脱毒苗的检验。...

Full description

Saved in:
Bibliographic Details
Published in西南农业学报 Vol. 29; no. 12; pp. 2866 - 2870
Main Author 王建辉 刘建军 陈克玲 李洪雯 何建 关斌 何礼
Format Journal Article
LanguageChinese
Published 农业部西南地区园艺作物生物学与种质创制实验室,四川成都610066 2016
四川省农业科学院,四川成都610066
四川省农业科学院园艺研究所,四川成都610066%农业部西南地区园艺作物生物学与种质创制实验室,四川成都610066
Subjects
多重PCR
草莓
草莓轻型黄边病毒
荧光定量PCR
Multiplex PCR
Fragariax ananassa
荧光定量PCR
草莓
Strawberry mild yellow edge virus
Real time PCR
草莓轻型黄边病毒
多重PCR
Online AccessGet full text
ISSN1001-4829
DOI10.16213/j.cnki.scjas.2016.12.018

Cover

More Information
Summary:本文对草莓轻型黄边病毒（Strawberry mild yellow edge virus，SMYEV）的2种分子检测技术进行了比较研究。结果表明，多重PCR技术以草莓actin为内参基因，完全排除在实验中杂质与操作的影响。Real time PCR技术具有更高的灵敏度和可靠性，可以定量检测出样本中病毒粒子的含量。结果表明，Real time PCR的灵敏度是普通PCR的100倍以上，而多重PCR的检测成本更经济。所以Real time PCR技术将用于引进品种的检疫，而多重PCR技术将用于大规模的脱毒苗的检验。
Bibliography:51-1213/S
Fragariax ananassa ;Strawberry mild yellow edge virus;Multiplex PCR;Real time PCR
WANG Jian-hui1,2, LIU Jian-jun1,3, , CHEN Ke-ling1,2, LI Hong-wen1,2, HE Jian1,2, GUAN Bin1,2 , HE Li1,2 （ 1. Horticulture Institute, Sichuan Academy of Agricultural Sciences, Sichuan Chengdu 610066, China; 2. Key Laboratory of Horticultural Crops Biology and Germplasm Enhancement in Southwest Regions, Ministry of Agriculture, Sichuan Chengdu 610066, China）
The comparative studies of two detection techniques on Strawberry mild yellow edge virus （SMYEV） were performed, respectively. Using actin of Fragariax ananassa as internal reference, multiplex PCR technique was used to detect SMYEV to avoid influencing by contaminants and mistakes. However, real time PCR was more sensitive and reliable detection on SMYEV quantitatively. The detection sensitivity of real time PCR is more than 100 folds than that of conventional PCR. But the multiplex PCR is more economic detection technique than real time PCR. So that real time PCR is
ISSN:1001-4829
DOI:10.16213/j.cnki.scjas.2016.12.018