油橄榄品种表型和SSR标记的多样性及聚类分析

[目的]利用表型性状和分子标记，对我国油橄榄品种进行鉴定和遗传多样性研究，有利于种质资源保存和利用，对了解油橄榄品种的组成结构及未来的引种和育种工作都具有重要意义。[方法]以甘肃省陇南市17个油橄榄品种为研究材料，利用表型上15个数量性状、18个质量性状和8个SSR荧光标记分别进行多样性和聚类分析。[结果]各性状在品种问存在显著差异，数量性状和质量性状的表型多样性指数分别介于1．579～2．089和0．362～1．091，8个SSR位点共检测到51个等位基因变异，平均每个位点等位基因数为6．375个，利用表型性状和SSR标记可以将17个品种完全区分开。[结论]17个油橄榄品种具有较丰富的表型和...

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Bibliographic Details
Published in林业科学研究 Vol. 29; no. 5; pp. 676 - 681
Main Author 秦倩 王楠楠 李金花 苏光灿
Format Journal Article
LanguageChinese
Published 林木遗传育种国家重点实验室国家林业局林木培育重点实验室中国林业科学研究院林业研究所,北京,100091%凉山州中泽新技术开发有限责任公司,四川西昌,615000 2016
Subjects
SSR荧光标记
油橄榄
聚类分析
表型性状
遗传多样性
Olea europaea L
genetic diversity
SSR
表型性状
遗传多样性
phenotypic data
SSR荧光标记
油橄榄
聚类分析
clustering analysis
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ISSN1001-1498

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Summary:[目的]利用表型性状和分子标记，对我国油橄榄品种进行鉴定和遗传多样性研究，有利于种质资源保存和利用，对了解油橄榄品种的组成结构及未来的引种和育种工作都具有重要意义。[方法]以甘肃省陇南市17个油橄榄品种为研究材料，利用表型上15个数量性状、18个质量性状和8个SSR荧光标记分别进行多样性和聚类分析。[结果]各性状在品种问存在显著差异，数量性状和质量性状的表型多样性指数分别介于1．579～2．089和0．362～1．091，8个SSR位点共检测到51个等位基因变异，平均每个位点等位基因数为6．375个，利用表型性状和SSR标记可以将17个品种完全区分开。[结论]17个油橄榄品种具有较丰富的表型和遗传多样性，基于表型数量性状、质量性状和SSR标记的聚类分析结果，判别品种间遗传关系的结果有一致的，也存在一定差异。对于表型性状相似的品种，需结合SSR标记进行品种鉴定。
Bibliography:11-1221/S
Olea europaea L. , phenotypic data, SSR, genetic diversity, clustering analysis
[ Objective ] With genetic markers and phenotypic data to identify olive cuhivars and evaluation their ge- netic diversity. [ Method ] Taking 11 olive cultivars from Longnan, Gansu Province, as samples, the genetic diversity analysis and cluster analysis were conducted with 15 quantitative traits, 18 quality phenotypic traits and 8 SSR markers. [ Result ] For all the phenotypic traits, significant variability was found among the cuhivars. The phenotypic diversity index of quantitative traits ranged from 1. 579 to 2. 088 as those of quality traits ranged from 0. 362 to 1.091. A total of 51 alleles were detected for 8 SSR markers, while the number of alleles per locus reached an average of 5.9. With the phenotypic traits and SSR markers, 17 cuhivars can be completely identified and distinguished. [ Conclusion ] The result of genetic distance analysis based on phenotypic traits and SSR markers showed similar genetic relations
ISSN:1001-1498