姜黄素对戊四氮致癫大鼠海马区IL-2和IL-6表达水平的影响

目的探讨姜黄素抗癫的作用机制。方法取健康成年雄性SD大鼠,连续腹腔注射戊四氮,诱发大鼠点燃致癫模型。空白组和模型组灌予生理盐水5ml,1次/d,连续28d。低剂量和高剂量姜黄素组分别灌予姜黄素100mg/kg和200mg/kg,1次/d,连续28d;丙戊酸钠组灌予丙戊酸钠400mg/kg,1次/d,连续28d。治疗结束后,按照Racine的6级评分标准,观察癫大鼠发作等级变化,用酶联免疫法（ELISA）检测海马区IL-2和IL-6表达水平的变化。结果姜黄素组的癫大鼠惊厥发作等级降低。模型组IL-2和IL-6高表达,比空白组呈显著升高（P〈0.05）。与模型组比较,姜黄素组大鼠海马区IL-2和I...

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Published in药学实践杂志 Vol. 34; no. 4; pp. 354 - 356
Main Author 董伟 严建维 谈巧玲 叶森
Format Journal Article
LanguageChinese
Published 余姚市人民医院,浙江余姚,315400 2016
Subjects
IL-2
IL-6
姜黄素
癫
epilepsy
IL-2
癫
姜黄素
IL-6
curcumin
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ISSN1006-0111
DOI10.3969/j.issn.1006-0111.2016.04.018

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Summary:目的探讨姜黄素抗癫的作用机制。方法取健康成年雄性SD大鼠,连续腹腔注射戊四氮,诱发大鼠点燃致癫模型。空白组和模型组灌予生理盐水5ml,1次/d,连续28d。低剂量和高剂量姜黄素组分别灌予姜黄素100mg/kg和200mg/kg,1次/d,连续28d;丙戊酸钠组灌予丙戊酸钠400mg/kg,1次/d,连续28d。治疗结束后,按照Racine的6级评分标准,观察癫大鼠发作等级变化,用酶联免疫法（ELISA）检测海马区IL-2和IL-6表达水平的变化。结果姜黄素组的癫大鼠惊厥发作等级降低。模型组IL-2和IL-6高表达,比空白组呈显著升高（P〈0.05）。与模型组比较,姜黄素组大鼠海马区IL-2和IL-6的表达明显降低（P〈0.05）;与低剂量姜黄素组相比,高剂量姜黄素组IL-2和IL-6水平降低更为明显（P〈0.05）。高剂量姜黄素组与丙戊酸钠组比较,无显著差异（P〉0.05）。结论姜黄素能降低癫大鼠惊厥发作级别,具有一定的抗癫作用,并且呈剂量依赖性,其可能机制是通过降低海马区IL-2和IL-6的表达发挥作用。
Bibliography:31-1685/R
DONG Wei, YAN Jianwei,TAN Qiaoling, YE Sen （Yuyao t eople＇s Hospital, Yuyao 315400, China）
DONG Wei;YAN Jianwei;TAN Qiaoling;YE Sen;Yuyao People′s Hospital; Objective To investigate the mechanism of anti-epileptic effect of the curcumin.Methods The SD rats were injected intraperitoneally with pentylenetetrazol kindling 25.0mg/kg to induce a rat epilepsy model.All of the treatments were performed once a day continuously for 28 days.The rats in blank group and model group received 5ml of normal saline.The rats in the high and low curcumin group were given 200mg/kg and 100mg/kg of curcumin once a day,respectively.The rats in the sodium valproate（VPA）group were given 400mg/kg of VPA once a day by gavage.After treatment,the seizures level was recorded by using the Racine′s six point grading scale,and the expression of IL-2and IL-6of hippocampus were detected by the enzyme linked immunoassay（ELISA）.Results The seizures level was reduced by curcumin in epileptic rats.The expressions of IL-2and IL-6of the mod
ISSN:1006-0111
DOI:10.3969/j.issn.1006-0111.2016.04.018