非小细胞肺癌外周血游离DNA及肿瘤细胞EGFR基因突变检测方法的研究进展

非小细胞肺癌（non-small cell lung cancer,NSCLC）是肺癌中最常见的类型。表皮生长因子受体（epidermal growth factor receptor,EGFR）酪氨酸激酶受体抑制剂（tyrosine kinases inhibitors,TKIs）是目前最重要的靶向治疗药物,对于有EGFR敏感突变的NSCLC,采用EGFR-TKIs治疗能取得明显的临床疗效;T790M突变是最常见的EGFR-TKIs耐药机制,通过对外周血进行EGFR基因突变检测,可以筛选出EGFR-TKIs治疗有效或治疗过程中对其产生耐药的患者。其定量分析不仅在肿瘤的早期诊断中具有重要意义,...

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Published in中国肺癌杂志 Vol. 19; no. 11; pp. 766 - 772
Main Author 张雨 徐燕 王孟昭
Format Journal Article
LanguageChinese
Published 100730北京,中国医学科学院,北京协和医学院,北京协和医院呼吸内科 2016
Subjects
EGFR
外周血
肺肿瘤
Plasma
肺肿瘤
EGFR
外周血
Lung neoplasms
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ISSN1009-3419
1999-6187
DOI10.3779/j.issn.1009-3419.2016.11.08

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Summary:非小细胞肺癌（non-small cell lung cancer,NSCLC）是肺癌中最常见的类型。表皮生长因子受体（epidermal growth factor receptor,EGFR）酪氨酸激酶受体抑制剂（tyrosine kinases inhibitors,TKIs）是目前最重要的靶向治疗药物,对于有EGFR敏感突变的NSCLC,采用EGFR-TKIs治疗能取得明显的临床疗效;T790M突变是最常见的EGFR-TKIs耐药机制,通过对外周血进行EGFR基因突变检测,可以筛选出EGFR-TKIs治疗有效或治疗过程中对其产生耐药的患者。其定量分析不仅在肿瘤的早期诊断中具有重要意义,而且是疗效评价及随访的重要生物学指标。目前,用于检测外周血EGFR突变的方法有很多,其中以在数字PCR基础上建立起来的dd PCR灵敏度最高,实现了对样本的高通量检测,定量程度较其他方法更为精确,在临床基因诊断中具有广阔的应用前景。
Bibliography:Yu ZHANG, Yah XU, Mengzhao WANG （Department of Respiratory Medicine, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China）
12-1395/R
Lung neoplasms; Plasma; EGFR
Non-small cell lung cancer（NSCLC） is the most common type of lung cancer. Epideral growth factor receptor tyrosine kinase inhibitors（EGFR-TKIs） are the most important treatments currently for advanced NSCLC patients harboring activating EGFR gene mutations, and achieve significant clinical efficacy. T790 M mutation occurs in half of NSCLC patents with acquired EGFR-TKI resistance. Screening for EGFR gene mutations in histological and/or circulating tumor cell or DNA samples of NSCLC patients can identify patients who would have a response to EGFR-TKIs or acquire resistance during the treatment. Quantitative analysis of plasma EGFR mutation is of great importance not only in early diagnosis of tumors, but also in curative effect evaluation and for follow-up. However, a strict requ
ISSN:1009-3419
1999-6187
DOI:10.3779/j.issn.1009-3419.2016.11.08