高效液相色谱法测定康得灵胶囊中黄芩苷的含量
目的：建立康得灵胶囊中黄芩苷的 HPLC测定方法。方法色谱柱为 Agilent Tc唱C18色谱柱（4．6 mm ×250 mm ，5μm），柱温为30℃；流动相为乙腈唱0．5‰磷酸溶液（26∶74），流速为1．0 ml／min ，检测波长265 nm。结果黄芩苷保留时间约为16 min。以峰面积（Y）对进样浓度（X ，μg／ml）线性回归，得回归方程：Y ＝22114．67X－112836．7，r＝0．9988，线性范围5．410-108．2μg／ml。平均加样回收率为98．78％，RSD为0．74％。结论本方法操作简便，测定结果准确可靠，可用于康得灵胶囊中黄芩苷的含量测定。...
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| Published in | 药学实践杂志 Vol. 34; no. 5; pp. 447 - 449 |
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| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
解放军208医院461临床部,吉林 长春,130021%空军航空大学门诊部,吉林长春,130022
2016
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1006-0111 |
| DOI | 10.3969/j.issn.1006-0111.2016.05.017 |
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| Summary: | 目的：建立康得灵胶囊中黄芩苷的 HPLC测定方法。方法色谱柱为 Agilent Tc唱C18色谱柱（4．6 mm ×250 mm ，5μm），柱温为30℃；流动相为乙腈唱0．5‰磷酸溶液（26∶74），流速为1．0 ml／min ，检测波长265 nm。结果黄芩苷保留时间约为16 min。以峰面积（Y）对进样浓度（X ，μg／ml）线性回归，得回归方程：Y ＝22114．67X－112836．7，r＝0．9988，线性范围5．410-108．2μg／ml。平均加样回收率为98．78％，RSD为0．74％。结论本方法操作简便，测定结果准确可靠，可用于康得灵胶囊中黄芩苷的含量测定。 |
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| Bibliography: | 31-1685/R JIANG Shuwen1 , LIU Jieping1 , XIAO Qiusheng1 , XIAO Jinbao1, SHI Chunshan1 , SUN Dan2 , JIANG Zongwen1 （1. Department of 461 Clinic, No. 208 Hospital of PLA, Changchun 130021,China; 2. Clinic of Navigate Air Force University, Changchun 130022, China） baicalin; HPLC Objective To establish an HPLC quantitative method for the content determination of baicalin in Kangdeling capsule .Methods The chromatography column was Agilent Tc-C18-WR （4 .6 mm× 250 mm ,5μm） ,and the column temper-ature was 30 ℃ .The mobile phase consisted of acetonitrile and 0 .5‰ phosphoric acid （26 ∶ 74 ） .The flow rate was 1 .0 ml/min ,and the detection wavelength was 265 nm .Results The retention time of baicalin was about 16 min .The calibra-tion equation was Y=22 114 .67 X -112 836 .7（r=0998 8）with good linearity in the range of 5 .410-108 .2 μg/ml for baicalin . The average recovery was 98 .78% while RSD were 0 .74% .Conclusion This method is simple ,time-saving and accurate which could be used to routine analysis of baicalin in |
| ISSN: | 1006-0111 |
| DOI: | 10.3969/j.issn.1006-0111.2016.05.017 |