禾花鲤致病性铜绿假单胞菌的分离鉴定及药敏试验

探明广西桂林市全州县禾花鲤暴发性死亡的病原菌及其药敏特性,为有效防控禾花鲤暴发性死亡现象提供依据。按常规方法进行病原菌的分离,人工感染试验确定病原菌,分别以API 20NE和16S rRNA基因序列分析对病原菌进行生化鉴定和分子鉴定,K-B纸片扩散法进行药敏试验。结果表明,从患病禾花鲤中分离到1株优势菌株TH4,该菌株对禾花鲤的平均致死率为80.00%,是引起禾花鲤暴发性死亡的病原菌;API 20NE和16S rRNA鉴定结果,TH4为铜绿假单胞菌（Pseudomonas aeruginosa）,与标准株Pseudomonas aeruginosa PA94（KM013815）的亲缘关系最近,...

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Published in西南农业学报 Vol. 29; no. 4; pp. 988 - 992
Main Author 龙苏 梁静真 韩书煜 范华龙 牛志伟 邓小红 胡大胜 黄钧
Format Journal Article
LanguageChinese
Published 广西大学动物科学技术学院/广西水生动物病害诊断实验室/广西高校水生生物健康养殖与营养调控重点实验室,广西南宁,530005%广西水产技术推广总站,广西南宁,530022%全州县水产技术推广站,广西全州,541500 2016
Subjects
病原分离与鉴定
禾花鲤
铜绿假单胞菌
禾花鲤
铜绿假单胞菌
Isolation and identification of pathogen
Pseudomonas aeruginosa
病原分离与鉴定
Procypris merus
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ISSN1001-4829
DOI10.16213/j.cnki.scjas.2016.04.046

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Summary:探明广西桂林市全州县禾花鲤暴发性死亡的病原菌及其药敏特性,为有效防控禾花鲤暴发性死亡现象提供依据。按常规方法进行病原菌的分离,人工感染试验确定病原菌,分别以API 20NE和16S rRNA基因序列分析对病原菌进行生化鉴定和分子鉴定,K-B纸片扩散法进行药敏试验。结果表明,从患病禾花鲤中分离到1株优势菌株TH4,该菌株对禾花鲤的平均致死率为80.00%,是引起禾花鲤暴发性死亡的病原菌;API 20NE和16S rRNA鉴定结果,TH4为铜绿假单胞菌（Pseudomonas aeruginosa）,与标准株Pseudomonas aeruginosa PA94（KM013815）的亲缘关系最近,同源相似度99.9%;病原菌TH4对菌必治等7种药物高度敏感,对青霉素G等7种药物耐药。
Bibliography:Procypris merus; Pseudomonas aeruginosa; Isolation and identification of pathogen
51-1213/S
In order to provide reference for effective prevention and control of explosive death,the present experiment was conducted to investigate pathogen of explosive death on Procypris merus cultured in Quanzhou,Guilin,Guangxi and drug sensitivity of pathogen. The pathogen was isolated by using conventional methods,then an artificial infection test was measured on the pathogenicity of the isolated strain. Biochemical and molecular identification of the pathogen was finished by API 20 NE analysis and 16 S rRNA gene sequencing,respectively. Moreover,the K-B paper diffusion method was used to test drug sensitivity of pathogen. Results showed that a dominant strain TH4 was isolated from diseased P. merus. And the average mortality of P. merus caused by the strain was 80. 00 %,which indicated that the strain was the pathogen of explosive death on P. merus. According to the results of API 20 NE analysis and 16 S rRNA gene sequencing
ISSN:1001-4829
DOI:10.16213/j.cnki.scjas.2016.04.046