油茶组培苗不定根形成过程酶的活性变化分析

【目的】分析油茶组培苗不定根发生过程中酶的变化规律,为油茶种苗培育提供参考依据。【方法】以广西主栽油茶无性系岑软2号油茶、桂无1号油茶和桂普24号油茶组培苗为材料,测定其生根过程中过氧化物酶（POD）、多酚氧化酶（PPO）和吲哚乙酸氧化酶（IAAO）活性。【结果】岑软2号和桂无1号油茶组培苗易生根,培养至50 d生根率大于84.0%,而桂普24号油茶组培苗培养至50 d生根率仅52.7%,属难生根无性系。在组培生根过程中,岑软2号和桂无1号油茶的POD、PPO和IAAO活性在根原基诱导期均呈急剧上升的变化趋势,在根原基形成约20 d达最高峰;桂普24号油茶的POD活性在整个生根期变化不明显,P...

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Published in南方农业学报 Vol. 47; no. 8; pp. 1344 - 1348
Main Author 陈晓明 韦璐阳 蔡玲 陈博雯 王鹏良 刘海龙
Format Journal Article
LanguageChinese
Published 广西林业科学研究院/国家林业局中南速生材繁育实验室/广西优良用材林资源培育重点实验室,南宁,530002%广西亚热带作物研究所,南宁,530001 2016
Subjects
不定根
吲哚乙酸氧化酶
多酚氧化酶
油茶
组培苗
过氧化物酶
油茶
组培苗
adventitious root
peroxidase
过氧化物酶
Camellia oleifera Abel
tissue culture plantlet
多酚氧化酶
polyphenol oxidase
indoleacetic acid oxidase
不定根
吲哚乙酸氧化酶
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ISSN2095-1191
DOI10.3969/j:issn.2095-1191.2016.08.1344

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Summary:【目的】分析油茶组培苗不定根发生过程中酶的变化规律,为油茶种苗培育提供参考依据。【方法】以广西主栽油茶无性系岑软2号油茶、桂无1号油茶和桂普24号油茶组培苗为材料,测定其生根过程中过氧化物酶（POD）、多酚氧化酶（PPO）和吲哚乙酸氧化酶（IAAO）活性。【结果】岑软2号和桂无1号油茶组培苗易生根,培养至50 d生根率大于84.0%,而桂普24号油茶组培苗培养至50 d生根率仅52.7%,属难生根无性系。在组培生根过程中,岑软2号和桂无1号油茶的POD、PPO和IAAO活性在根原基诱导期均呈急剧上升的变化趋势,在根原基形成约20 d达最高峰;桂普24号油茶的POD活性在整个生根期变化不明显,PPO活性明显低于岑软2号和桂无1号油茶,IAAO活性前期不断降低,有利于吲哚乙酸（IAA）的累积,容易形成愈伤组织。【结论】油茶组培苗生根诱导前期提高其POD、PPO和IAAO活性,有利于根原基的诱导,生根诱导后期降低其POD、PPO和IAAO活性有利于不定根伸长。生产上培育油茶组培苗时在培养基中调整外源激素和外源酚等物质的用量可提高组培苗的生根率。
Bibliography:45-1381/S
[Objective]Tile present experiment was conducted to study change law of enzyme in the adventitious root formation of Camellia oleifera Abel. tissue-cultured plantlets, in order to provide theoretical basis for breeding C. oleifera plantlets. [Method]Using the tissue-culture plantlets of different C. oleifera clones viz., Cenruan 2, Guiwu 1 and Guipu 24 as materials, the activities of peroxidase （POD）, polyphenol oxidase （PPO） and indoleaeetic acid oxidase （IAAO） were de- termined in the rooting process. [Result]The tissue-cuhured plantlets of Cenruan 2 and Guiwu 1 were easy to root, their rooting rate were more than 84.0% after being cultured for 50 days, but the rooting rate of Guipu 24 was only 52.7%, so Guipu 24 was difficult to root. The POD, PPO and IAAO activities of Cenruan 2 and Guiwu 1 showed a sharp upward trend during the root primordium induction period, and which reached up to peak about 20 days after root primordium for- mation. Furthermore, the POD activity of Guipu 24 didn＇t change si
ISSN:2095-1191
DOI:10.3969/j:issn.2095-1191.2016.08.1344