Impaired Recall of Positional Memory following Chemogenetic Disruption of Place Field Stability

• Published in: Cell reports (Cambridge) Vol. 16; no. 3; pp. 793 - 804
• Main Authors: Zhao, Rong, Grunke, Stacy D., Keralapurath, Madhusudhanan M., Yetman, Michael J., Lam, Alexander, Lee, Tang-Cheng, Sousounis, Konstantinos, Jiang, Yongying, Swing, Deborah A., Tessarollo, Lino, Ji, Daoyun, Jankowsky, Joanna L.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 19.07.2016; Elsevier
• Subjects: Animals; Entorhinal Cortex - physiology; Female; Hippocampus - physiology; Humans; Male; Mice; Mice, Inbred C3H; Mice, Inbred C57BL; Models, Neurological; Nerve Net - physiology; Perforant Pathway - physiology; Spatial Memory - physiology; Temporal Lobe - physiology
• ISSN: 2211-1247; 2211-1247
• DOI: 10.1016/j.celrep.2016.06.032

The neural network of the temporal lobe is thought to provide a cognitive map of our surroundings. Functional analysis of this network has been hampered by coarse tools that often result in collateral damage to other circuits. We developed a chemogenetic system to temporally control electrical input into the hippocampus. When entorhinal input to the perforant path was acutely silenced, hippocampal firing patterns became destabilized and underwent extensive remapping. We also found that spatial memory acquired prior to neural silencing was impaired by loss of input through the perforant path. Together, our experiments show that manipulation of entorhinal activity destabilizes spatial coding and disrupts spatial memory. Moreover, we introduce a chemogenetic model for non-invasive neuronal silencing that offers multiple advantages over existing strategies in this setting. [Display omitted] •Transgenic model for spatiotemporal control of neuronal silencing via systemic ligand•Acute silencing of entorhinal cortex causes global disruption of CA1 spatial tuning•Concomitant with CA1 remapping, entorhinal silencing degrades spatial recall•Both place field stability and spatial memory require the same ongoing cortical input Zhao et al. present a chemogenetic model for acute neuronal silencing. Suppression of the entorhinal cortex causes remapping of hippocampal CA1 place fields and impairs recall of spatial memory. The concurrent disruption of place fields and spatial recall suggest that stable cognitive maps remain critical for navigation in a familiar setting.