Vein patterning screens and the defectively organized tributaries mutants in Arabidopsis thaliana

• Published in: The Plant journal : for cell and molecular biology Vol. 56; no. 2; pp. 251 - 263
• Main Authors: Petricka, Jalean Joyanne, Clay, Nicole Kho, Nelson, Timothy Mark
• Format: Journal Article
• Language: English
• Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01.10.2008; Blackwell Publishing Ltd; Blackwell Science
• Subjects: anatomy & histology; Arabidopsis; Arabidopsis - anatomy & histology; Arabidopsis - genetics; Arabidopsis Proteins; Arabidopsis Proteins - genetics; Arabidopsis thaliana; Biological and medical sciences; Botany; C2H2 zinc finger WIP domain; Chromosome Mapping; Chromosomes, Plant; Chromosomes, Plant - genetics; Cloning, Molecular; Computer simulation; dissociation; DNA, Plant; DNA, Plant - genetics; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Developmental; Gene Expression Regulation, Plant; genes; Genes, Plant; genetic screen; genetics; glycine-rich protein; Leaves; Multigene Family; Mutagenesis; Mutants; Mutation; NON-PHOTOTROPHIC HYPOCOTYL 3; ontogeny; pentatricopeptide repeat; Phenotype; photosynthates; Plant growth. Development of the storage organs; Plant Leaves; Plant Leaves - anatomy & histology; Plant Leaves - genetics; Plant physiology and development; plant veins; Plants; SART-1; Signal transduction; simulation models; spatial distribution; Vegetative apparatus, growth and morphogenesis. Senescence; vein patterning; Veins (plant anatomy); Genetic mapping; Phototrophy; Patterning; genetic screen; NON-PHOTOTROPHIC HYPOCOTYL 3; glycine-rich protein; SART-1; pentatricopeptide repeat; Plant leaf; Linkage equilibrium; Protein; Arabidopsis thaliana; Morphogenesis; Hypocotyl; Gene; vein patterning; Cruciferae; Dicotyledones; C2H2 zinc finger WIP domain; Angiospermae; Spermatophyta; Vein
• ISSN: 0960-7412; 1365-313X; 1365-313X
• DOI: 10.1111/j.1365-313X.2008.03595.x

Leaf veins form a closed network that transports essential photosynthates, water and signaling molecules to the developing plant. The formation of the patterns of these networks during leaf ontogeny is an active subject of modeling and computer simulation. To investigate the vein patterning process, we performed screens for defects in juvenile leaf vein patterning in Arabidopsis thaliana lines subjected to mutagenesis via diepoxybutane, activation tagging or the Dissociation/Activator transposon. We identified over 40 vein pattern defective lines, providing a phenotypic resource for the testing of vein patterning models. In addition, we report the chromosomal linkage for 13 of these, eight of which were successfully cloned. We further describe the phenotypes of five of these mutants, which we call the defectively organized tributaries (dot) mutants, and their corresponding molecular identities. The diversity of the individual genes affected in this collection of pattern mutants suggests that vein pattern is highly sensitive to perturbations in many cellular processes. Despite this diversity of causes, the resulting pattern defects fall into a limited number of classes, including parallel, spurred, misaligned, open, midvein gap and irregularly spaced. These classes may represent sensitivities to cellular processes associated with the DOT genes. The ontogeny of common defective patterns should be accommodated into any robust model for the ontogeny and evolution of pattern.