转抗旱基因IrrE玉米材料的获得及抗性鉴定

以优良玉米自交系综31的幼胚为受体材料，采用农杆菌介导法将来自微生物耐辐射奇球（Deinococcus radiodurans）的抗旱基因IrrE转入玉米幼胚组织细胞中，转化后的细胞通过抗性筛选，分化再生成苗。结果表明，经特异PCR检测，42株植株转入了，IrrE基因，有29株收获种子。T1代在温室大棚控制水肥试验及脯氨酸等生理生化指标检测表明，转基因植株具有良好的耐旱特性，为抗旱玉米新品种选育提供了较好的亲本材料。...

Full description

Saved in:
Bibliographic Details
Published in西南农业学报 Vol. 29; no. 8; pp. 1782 - 1786
Main Author 余桂容 张维 杜文平 宋军 陈谦 徐利远
Format Journal Article
LanguageChinese
Published 四川省农业科学院生物技术核技术研究所,四川成都,610066%中国农业科学院生物技术研究所,北京,100081 2016
Subjects
IrrE基因
抗旱鉴定
玉米幼胚
遗传转化
抗旱鉴定
Drought-resistance identification
遗传转化
Transgenic
IrrE gene
IrrE基因
Maize immature-embryo
玉米幼胚
Online AccessGet full text
ISSN1001-4829
DOI10.16213/j.cnki.scjas.2016.08.005

Cover

More Information
Summary:以优良玉米自交系综31的幼胚为受体材料，采用农杆菌介导法将来自微生物耐辐射奇球（Deinococcus radiodurans）的抗旱基因IrrE转入玉米幼胚组织细胞中，转化后的细胞通过抗性筛选，分化再生成苗。结果表明，经特异PCR检测，42株植株转入了，IrrE基因，有29株收获种子。T1代在温室大棚控制水肥试验及脯氨酸等生理生化指标检测表明，转基因植株具有良好的耐旱特性，为抗旱玉米新品种选育提供了较好的亲本材料。
Bibliography:51-1213/S
YU Gui-rong , ZHANG Wei, DU Wen-ping , SONG Jun , CHEN Qian, XU Li-yuan （ 1. Institute of Biotechnology ＆ Nuclear Techniques, Sichuan Academy of Agricultural Sciences, Siehuan Chengdu 610066, China; 2. Biotechnology Research Institute, China Academy of Agricultural Sciences, Beijing 100081, China）
Maize immature-embryo; Transgenic ; Drought- resistance identification ; IrrE gene
Taken the maize inbred＇ Zhong-31 ＇ as the transgenie receptor, the drought- resistant gene（IrrE） from Deinococcus radiodurans by Agrobacterium - mediated was transformed into the immature-embryos of Zhong-31, its immature-embryos were screen out,differentiated and regenerated plants. The result showed that IrrE in 42 transgenic plants fi＇om initial regenerated plants by PCR detection were identified, of which the seeds of their 29 plants were harvested. Based on the regulation of water and nutrients, the analysis of proline, and so on for Tt , the resuhs indicated that transgenic plants had well drought-resisting characters, wh
ISSN:1001-4829
DOI:10.16213/j.cnki.scjas.2016.08.005