几种新疆加工型辣椒种子病毒的检测和鉴定

本研究采用双抗体夹心酶联免疫吸附法（DAS-ELISA）对新疆巴音郭楞蒙古自治州和静地区9个品种的加工型辣椒种子样品进行9种主要病毒的检测。针对检测呈阳性的病毒,进一步通过反转录聚合酶链式反应（RT-PCR）,对其基因克隆和序列分析验证。结果表明,检测的辣椒种子中携带CMV、BBWV和PMMo V,以CMV的检出率最高,为55.6%,其次是BBWV,检出率为22.2%,PMMo V的检出率最低,为11.1%。CMV不仅是种子携带的优势毒源,且与BBWV和PMMo V复合侵染。种子传毒试验表明其种子传毒率为4.7%～17.4%。研究发现,辣椒种植地域、辣椒品种（种类）影响种子携带病毒种类、带毒率...

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Published in西南农业学报 Vol. 27; no. 6; pp. 2385 - 2392
Main Author 张强 罗明 李克梅 张春竹 楚金平 马建江
Format Journal Article
LanguageChinese
Published 新疆自治区高校农林有害生物监测与安全防控重点实验室,新疆乌鲁木齐830052%新疆巴音郭楞蒙古自治州农业科学研究所,新疆库尔勒,841000%新疆库尔勒市农业技术推广中心,新疆库尔勒,841000 2014
新疆农业大学农学院,新疆乌鲁木齐830052
Subjects
DAS-ELISA
RT-PCR
病毒
辣椒种子
DAS-ELISA
Virus
病毒
seed
Pepper
辣椒种子
RT-PCR
Online AccessGet full text
ISSN1001-4829
DOI10.16213/j.cnki.scjas.2014.06.025

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Summary:本研究采用双抗体夹心酶联免疫吸附法（DAS-ELISA）对新疆巴音郭楞蒙古自治州和静地区9个品种的加工型辣椒种子样品进行9种主要病毒的检测。针对检测呈阳性的病毒,进一步通过反转录聚合酶链式反应（RT-PCR）,对其基因克隆和序列分析验证。结果表明,检测的辣椒种子中携带CMV、BBWV和PMMo V,以CMV的检出率最高,为55.6%,其次是BBWV,检出率为22.2%,PMMo V的检出率最低,为11.1%。CMV不仅是种子携带的优势毒源,且与BBWV和PMMo V复合侵染。种子传毒试验表明其种子传毒率为4.7%～17.4%。研究发现,辣椒种植地域、辣椒品种（种类）影响种子携带病毒种类、带毒率和种传率。本研究结果是新疆加工型辣椒种子病毒首次报道。
Bibliography:51-1213/S
ZHANG Qiang,LUO Ming,LI Ke-mei,ZHANG Chun-zhu,CHU Jin-ping,MA Jian-jiang（ 1. Agricultural College of Xinjiang Agricultural University,Xinjiang Urumqi 830052 ,China;2. Key Laboratory of Xinjiang Autonomous Region Universities Forestry Harmful Biological Monitoring and Safety Prevention, Xinjiang Urumqi 830052, China;3. Institute of Agricultural Sciences of Bayinguoleng Mongol Autonomous Prefecture in Xinjiang, Xinjiang Korla 841000, China;4. Agricultural Technology Promotion Center of Korla in Xinjiang, Xinjiang Korla 841000, China）
In this study,9 main viruses which came from 9 varieties of processing pepper seed samples were collected at Hejing in Bayinguoleng Mongolia Autonomous Region of Xinjiang were detected by double antibody sandwich enzyme-linked immunosorbent assay（ DASELISA）. In the light of the virus＇ test which showed positive,using reverse transcription polymerase chain reaction（ RT-PCR）,their gene cloning and sequence were analyzed and verified. The results showed that among the detected
ISSN:1001-4829
DOI:10.16213/j.cnki.scjas.2014.06.025