蓝莓品种阳光蓝离体繁殖条件的筛选

【目的】筛选适宜蓝莓品种阳光蓝（Sunshine Blue）的离体繁殖条件，建立阳光蓝组培快繁体系。【方法】以阳光蓝无菌苗茎段为外植体进行离体繁殖，筛选适合其芽增殖及生根的培养基类型、茎段起始芽数、细胞分裂素、生长素、pH及活性炭（AC）添加量。【结果】阳光蓝茎段在WPM培养基中增殖芽数最多，培养60 d时平均芽长较长，可作为阳光蓝茎段增殖较佳的基本培养基；以起始芽数2芽茎段接种到WPM＋2.0 mg/L ZT＋0.01 mg/L IBA培养基上，培养30 d时增殖芽数为10.25个，显著多于其他增殖培养基（P〈0.05），培养60 d时增殖芽数达17.43个，增殖效果明显优于其他增殖培养基。...

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Bibliographic Details
Published in南方农业学报 Vol. 47; no. 11; pp. 1897 - 1902
Main Author 范淑芳 筒大为 周志翔 宁国贵
Format Journal Article
LanguageChinese
Published 荆楚理工学院 生物工程学院,湖北 荆门,448000%荆门市林业局，湖北 荆门 448000 2016
华中农业大学 园艺林学学院，武汉 430070%华中农业大学 园艺林学学院,武汉,430070
Subjects
条件筛选
离体繁殖
蓝莓
阳光蓝
Vaccinium spp
离体繁殖
条件筛选
蓝莓
condition screening
in vitro propagation
阳光蓝
Sunshine Blue
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ISSN2095-1191
DOI10.3969/jissn.2095-1191.2016.11.1897

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Summary:【目的】筛选适宜蓝莓品种阳光蓝（Sunshine Blue）的离体繁殖条件，建立阳光蓝组培快繁体系。【方法】以阳光蓝无菌苗茎段为外植体进行离体繁殖，筛选适合其芽增殖及生根的培养基类型、茎段起始芽数、细胞分裂素、生长素、pH及活性炭（AC）添加量。【结果】阳光蓝茎段在WPM培养基中增殖芽数最多，培养60 d时平均芽长较长，可作为阳光蓝茎段增殖较佳的基本培养基；以起始芽数2芽茎段接种到WPM＋2.0 mg/L ZT＋0.01 mg/L IBA培养基上，培养30 d时增殖芽数为10.25个，显著多于其他增殖培养基（P〈0.05），培养60 d时增殖芽数达17.43个，增殖效果明显优于其他增殖培养基。在生根培养中，当生长素为0.10 mg/L IBA、添加1000.0 mg/L AC、培养90 d时生根率达87.50%，明显优于其他生根培养基。【结论】阳光蓝组培苗的离体繁殖以带2个芽茎段在WPM＋2.0 mg/L ZT＋0.01 mg/L IBA中进行增殖培养、在1/2WPM＋0.10 mg/L IBA＋1000.0 mg/L AC中进行生根培养效果较佳。
Bibliography:Vaccinium spp. ; Sunshine Blue ; in vitro propagation ; condition screening
FAN Shu-fang1,JIAN Da-wei2,3, ZHOU Zhi-xiang 3, NING Guo-gui3 （1College of Biological Engineering, Jingchu University ofTeehnology, Jingmen, Hubei 448000, China; 2Jingmen Forestry Bureau, Jingmen, Hubei 448000, China; ZCollege of Hortieuhure ＆ Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China）
45-1381/S
Objective]The present study was conducted to screen in vitro propagation condition for Vaccinium spp. Sunshine Blue in order to establish a rapid propagation system for blueberry Sunshine Blue. [Method]In this experiment, using Sunshine Blue’s aseptic seedling stems as explants, proper medium, initial shoot number, cytokinins, auxins, pH value and activated carbon（AC） content which were suitable for shoot proliferation and rooting were screened. [Result]At shoot proliferation stage,the stems cultured in WPM medium had the largest number of proliferation shoots and the average shoot length was long after 60 days of c
ISSN:2095-1191
DOI:10.3969/jissn.2095-1191.2016.11.1897