优良木薯品种新选048离体快繁技术

【目的】探讨木薯新品种新选048的离体快速繁殖技术，为扩大种苗生产提供参考。【方法】以带腋芽的幼嫩茎段为外植体，研究不同季节取材、不同灭菌方法、不同用量植物生长调节剂以及不同培养方式对新选048离体快繁过程的影响。【结果】相对于冬季、夏季，春季采集的腋芽萌发快、生长势旺，污染率为22．1％，成活率为69．3％。在8～12min内，随着0．1％HgCl2、2．0％NaCl0灭菌时间的延长，外植体污染率降低，但褐化率随之升高；不同处理中，以0．1％HgCl2处理10min对外植体的灭菌效果较好。在继代增殖培养基中，随着6-BA用量（0．02～1．00mg／L）的升高，诱导的愈伤组织长度、鲜重和干重...

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Bibliographic Details
Published in南方农业学报 Vol. 45; no. 6; pp. 950 - 954
Main Author 曾文丹 陆柳英 谢向誉 周慧文 严华兵
Format Journal Article
LanguageChinese
Published 广西作物遗传改良生物技术重点开放实验室,南宁530007%广西农业科学院经济作物研究所,南宁,530007%广西大学农学院,南宁,530005 2014
广西农业科学院经济作物研究所,南宁530007
Subjects
快速繁殖
新选048
木薯
组织培养
腋芽
腋芽
组织培养
Xinxuan 048
tissue culture
cassava
axillary bud
木薯
rapid propagation
新选048
快速繁殖
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ISSN2095-1191
DOI10.3969/j:issn.2095-1191.2014.6.950

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Summary:【目的】探讨木薯新品种新选048的离体快速繁殖技术，为扩大种苗生产提供参考。【方法】以带腋芽的幼嫩茎段为外植体，研究不同季节取材、不同灭菌方法、不同用量植物生长调节剂以及不同培养方式对新选048离体快繁过程的影响。【结果】相对于冬季、夏季，春季采集的腋芽萌发快、生长势旺，污染率为22．1％，成活率为69．3％。在8～12min内，随着0．1％HgCl2、2．0％NaCl0灭菌时间的延长，外植体污染率降低，但褐化率随之升高；不同处理中，以0．1％HgCl2处理10min对外植体的灭菌效果较好。在继代增殖培养基中，随着6-BA用量（0．02～1．00mg／L）的升高，诱导的愈伤组织长度、鲜重和干重均不断增加，而不定芽的增殖系数、组培苗的鲜重和干重呈先升高后降低的变化趋势，但不定芽高度逐渐降低；以添加0．05mg／L6-BA的增殖效果最好，最高增殖系数为6．1倍，组培苗鲜重和干重综合表现较好。采用固体培养基培养的木薯不定芽增殖系数和组培苗干重最高，且诱导的不定芽长势均一、生长势旺；采用液体培养基培养的不定芽株高和组培苗鲜重最高，而间歇式浸没培养诱导的木薯不定芽增殖系数、株高、组培苗鲜重和干重均较低。在1／3MS＋0．02mg／LNAA＋20．0g/L蔗糖＋6．0g/L琼脂粉的生根培养基中不定芽生根率达100．0％，根多且长，呈乳白色。【结论】成功建立了木薯品种新选048以带腋芽的茎段为外植体的快速繁殖体系，可为其种苗生产提供新的途径。
Bibliography:45-1381/S
[Objective]The present study was conducted to develop rapid propagation technology of cassava variety Xinxuan 048 in order to provide references for expanding its seed-using seedling prodution. [Method]The young stems with axillary bud was used as explants, the effects of different sampling seasons, sterilization methods, various concentra- tion of 6-BA and different culture media on the process of plautlets culture were investigated. [Result]As compared with samling in winter and summer, the axillary buds of stem samped in spring germinated lastly and grew well, it had low contamination rate of 22.1% and higher survival rate of 69.3%. In the range of 8-12 min, the explants showed decreasing contamination rate and increasing browning rate with prolonging sterilizing time of 0.1% HgCl2 and 2.0% NaC10, and the sterilizing effects was found the best by sterilization of 0.1% HgCl2 for 10 min. In the process of subculture, with the increase of 6-BA（0.02-1.00 mg/L）, the induced callus presented increasing
ISSN:2095-1191
DOI:10.3969/j:issn.2095-1191.2014.6.950