3种东方百合离体培养
【目的】研究3种东方百合离体培养技术,筛选出适宜其离体培养的统一培养基,为东方百合工厂化育苗提供技术保障。【方法】以3种东方百合提伯、索邦和西伯利亚的鳞片为外植体,筛选适宜的灭菌方法;在MS基本培养基上添加不同种类和不同浓度的激素,筛选适宜的增殖培养基和生根培养基,进一步筛选最适移栽基质。【结果】适宜3种东方百合外植体灭菌的较好方法是：75%酒精灭菌15 s结合升汞灭菌12 min;愈伤诱导和分化的适宜培养基为MS;MS＋0.5 mg/L 6-BA＋0.5 mg/L NAA是增殖培养的最适培养基,提伯、索邦和西伯利亚的增殖系数分别为3.32、3.32和3.00;MS＋0.1 mg/L IBA是...
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| Published in | 南方农业学报 Vol. 47; no. 6; pp. 889 - 894 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
广西农业科学院 花卉研究所,南宁,530007
2016
|
| Subjects | |
| Online Access | Get full text |
| ISSN | 2095-1191 |
| DOI | 10.3969/j:issn.2095-1191.2016.06.889 |
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| Abstract | 【目的】研究3种东方百合离体培养技术,筛选出适宜其离体培养的统一培养基,为东方百合工厂化育苗提供技术保障。【方法】以3种东方百合提伯、索邦和西伯利亚的鳞片为外植体,筛选适宜的灭菌方法;在MS基本培养基上添加不同种类和不同浓度的激素,筛选适宜的增殖培养基和生根培养基,进一步筛选最适移栽基质。【结果】适宜3种东方百合外植体灭菌的较好方法是：75%酒精灭菌15 s结合升汞灭菌12 min;愈伤诱导和分化的适宜培养基为MS;MS＋0.5 mg/L 6-BA＋0.5 mg/L NAA是增殖培养的最适培养基,提伯、索邦和西伯利亚的增殖系数分别为3.32、3.32和3.00;MS＋0.1 mg/L IBA是生根培养的最佳培养基,提伯、索邦和西伯利亚的生根率分别为96.33%、92.67%和93.67%;移栽的最佳基质为泥炭∶珍珠岩（体积比）=2∶1,提伯、索邦和西伯利亚的移栽成活率分别为97.34%、98.00%和100.00%。【结论】建立的3种东方百合离体快繁体系适用于东方百合种球工厂化生产。 |
|---|---|
| AbstractList | S682.2; 【目的】研究3种东方百合离体培养技术，筛选出适宜其离体培养的统一培养基，为东方百合工厂化育苗提供技术保障。【方法】以3种东方百合提伯、索邦和西伯利亚的鳞片为外植体，筛选适宜的灭菌方法；在MS基本培养基上添加不同种类和不同浓度的激素，筛选适宜的增殖培养基和生根培养基，进一步筛选最适移栽基质。【结果】适宜3种东方百合外植体灭菌的较好方法是：75%酒精灭菌15 s结合升汞灭菌12 min；愈伤诱导和分化的适宜培养基为MS；MS+0.5 mg/L 6-BA+0.5 mg/L NAA是增殖培养的最适培养基，提伯、索邦和西伯利亚的增殖系数分别为3.32、3.32和3.00；MS+0.1 mg/L IBA是生根培养的最佳培养基，提伯、索邦和西伯利亚的生根率分别为96.33%、92.67%和93.67%；移栽的最佳基质为泥炭∶珍珠岩（体积比）＝2∶1，提伯、索邦和西伯利亚的移栽成活率分别为97.34%、98.00%和100.00%。【结论】建立的3种东方百合离体快繁体系适用于东方百合种球工厂化生产。 【目的】研究3种东方百合离体培养技术,筛选出适宜其离体培养的统一培养基,为东方百合工厂化育苗提供技术保障。【方法】以3种东方百合提伯、索邦和西伯利亚的鳞片为外植体,筛选适宜的灭菌方法;在MS基本培养基上添加不同种类和不同浓度的激素,筛选适宜的增殖培养基和生根培养基,进一步筛选最适移栽基质。【结果】适宜3种东方百合外植体灭菌的较好方法是：75%酒精灭菌15 s结合升汞灭菌12 min;愈伤诱导和分化的适宜培养基为MS;MS＋0.5 mg/L 6-BA＋0.5 mg/L NAA是增殖培养的最适培养基,提伯、索邦和西伯利亚的增殖系数分别为3.32、3.32和3.00;MS＋0.1 mg/L IBA是生根培养的最佳培养基,提伯、索邦和西伯利亚的生根率分别为96.33%、92.67%和93.67%;移栽的最佳基质为泥炭∶珍珠岩（体积比）=2∶1,提伯、索邦和西伯利亚的移栽成活率分别为97.34%、98.00%和100.00%。【结论】建立的3种东方百合离体快繁体系适用于东方百合种球工厂化生产。 |
| Abstract_FL | Objective]The in vitro tissue culture technique of oriental lily was studied, and the unified culture medi-um was screened, in order to provide theoretical and technical support for industrialized seedling production. [Method]The bulb scales of three lily cultivars viz., Tiber, Sorbonne and Siberi were used as explants, which were cultured in minimal medium(MS) supplemented with different hormones. [Result]The results showed that, the best sterilization method was as follow: 0.1% HgCl2 for 12 min, followed by 75% alcohol for 15 s. The suitable culture medium for callus induction and differentiation was MS. The optimum medium for subculture multiplication was MS+0.5 mg/L6-BA+0.5 mg/L NAA , and the multiplication coefficients of Tiber, Sorbonne and Siberi were 3.32, 3.32 and 3.00, respectively. The optimum culture medium for rooting culture was MS+0.1 mg/L IBA, and the rooting rates of Tiber, Sorbonne and Siberi were 96.33%, 92.67%, 93.67% respectively. The optimum substrate for transplanting was peat mixed with perlite in the volume propor-tion of 2∶1, the survival rates of Tiber, Sorbonne and Siberi were 97.34%, 98.00% and 100.00%, respectively. [Conclu-sion]The tissue culture and rapid propagation system for 3 varieties of oriental lily is established , and which is applicable to factory production of lily bulb. |
| Author | 闫海霞 蒋月喜 邓杰玲 黄昌艳 王晓国 何荆洲 卜朝阳 |
| AuthorAffiliation | 广西农业科学院花卉研究所,南宁530007 |
| AuthorAffiliation_xml | – name: 广西农业科学院 花卉研究所,南宁,530007 |
| Author_FL | YAN Hai-xia JIANG Yue-xi DENG Jie-ling HE Jing-zhou BU Zhao-yang HUANG Chang-yan WANG Xiao-guo |
| Author_FL_xml | – sequence: 1 fullname: YAN Hai-xia – sequence: 2 fullname: JIANG Yue-xi – sequence: 3 fullname: DENG Jie-ling – sequence: 4 fullname: HUANG Chang-yan – sequence: 5 fullname: WANG Xiao-guo – sequence: 6 fullname: HE Jing-zhou – sequence: 7 fullname: BU Zhao-yang |
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| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
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| DocumentTitleAlternate | In vitro culture for 3 varieties of oriental lily |
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| Keywords | 离体培养 oriental lily 外植体 in vitro culture 东方百合 medium 培养基 explant |
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| Notes | 45-1381/S YAN Hai-xia ,JIANG Yue-xi, DENG Jie-ling, HUANG Chang-yan, WANG Xiao-guo, HE Jing-zhou, BU Zhao-yang （Flower Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China） [Objective]The in vitro tissue culture technique of oriental lily was studied, and the unified culture medi- um was screened, in order to provide theoretical and technical support for industrialized seedling production. [Method]The bulb scales of three lily cultivars viz., Tiber, Sorbonne and Siberi were used as explants, which were cultured in minimal medium（MS） supplemented with different hormones. [Result]The results showed that, the best sterilization method was as follow： 0.1% HgC12 for 12 min, followed by 75% alcohol for 15 s. The suitable cuhure medium for callus induction and differentiation was MS. The optimum medium for subculture multiplication was MS＋0.5 mg/L6-BA＋0.5 mg/L NAA, and the muhiplication coefficients of Tiber, Sorbonne and Siberi were 3.32, 3.32 and 3.00, respectively. The optimum culture |
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| Snippet | 【目的】研究3种东方百合离体培养技术,筛选出适宜其离体培养的统一培养基,为东方百合工厂化育苗提供技术保障。【方法】以3种东方百合提伯、索邦和西伯利亚的鳞片为外植体,筛选适... S682.2; 【目的】研究3种东方百合离体培养技术，筛选出适宜其离体培养的统一培养基，为东方百合工厂化育苗提供技术保障。【方法】以3种东方百合提伯、索邦和西伯利亚的鳞片为外... |
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| Title | 3种东方百合离体培养 |
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