3种东方百合离体培养

【目的】研究3种东方百合离体培养技术,筛选出适宜其离体培养的统一培养基,为东方百合工厂化育苗提供技术保障。【方法】以3种东方百合提伯、索邦和西伯利亚的鳞片为外植体,筛选适宜的灭菌方法;在MS基本培养基上添加不同种类和不同浓度的激素,筛选适宜的增殖培养基和生根培养基,进一步筛选最适移栽基质。【结果】适宜3种东方百合外植体灭菌的较好方法是：75%酒精灭菌15 s结合升汞灭菌12 min;愈伤诱导和分化的适宜培养基为MS;MS＋0.5 mg/L 6-BA＋0.5 mg/L NAA是增殖培养的最适培养基,提伯、索邦和西伯利亚的增殖系数分别为3.32、3.32和3.00;MS＋0.1 mg/L IBA是...

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Published in南方农业学报 Vol. 47; no. 6; pp. 889 - 894
Main Author 闫海霞 蒋月喜 邓杰玲 黄昌艳 王晓国 何荆洲 卜朝阳
Format Journal Article
LanguageChinese
Published 广西农业科学院 花卉研究所,南宁,530007 2016
Subjects
东方百合
培养基
外植体
离体培养
离体培养
oriental lily
外植体
in vitro culture
东方百合
medium
培养基
explant
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ISSN2095-1191
DOI10.3969/j:issn.2095-1191.2016.06.889

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Summary:【目的】研究3种东方百合离体培养技术,筛选出适宜其离体培养的统一培养基,为东方百合工厂化育苗提供技术保障。【方法】以3种东方百合提伯、索邦和西伯利亚的鳞片为外植体,筛选适宜的灭菌方法;在MS基本培养基上添加不同种类和不同浓度的激素,筛选适宜的增殖培养基和生根培养基,进一步筛选最适移栽基质。【结果】适宜3种东方百合外植体灭菌的较好方法是：75%酒精灭菌15 s结合升汞灭菌12 min;愈伤诱导和分化的适宜培养基为MS;MS＋0.5 mg/L 6-BA＋0.5 mg/L NAA是增殖培养的最适培养基,提伯、索邦和西伯利亚的增殖系数分别为3.32、3.32和3.00;MS＋0.1 mg/L IBA是生根培养的最佳培养基,提伯、索邦和西伯利亚的生根率分别为96.33%、92.67%和93.67%;移栽的最佳基质为泥炭∶珍珠岩（体积比）=2∶1,提伯、索邦和西伯利亚的移栽成活率分别为97.34%、98.00%和100.00%。【结论】建立的3种东方百合离体快繁体系适用于东方百合种球工厂化生产。
Bibliography:45-1381/S
YAN Hai-xia ,JIANG Yue-xi, DENG Jie-ling, HUANG Chang-yan, WANG Xiao-guo, HE Jing-zhou, BU Zhao-yang （Flower Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China）
[Objective]The in vitro tissue culture technique of oriental lily was studied, and the unified culture medi- um was screened, in order to provide theoretical and technical support for industrialized seedling production. [Method]The bulb scales of three lily cultivars viz., Tiber, Sorbonne and Siberi were used as explants, which were cultured in minimal medium（MS） supplemented with different hormones. [Result]The results showed that, the best sterilization method was as follow： 0.1% HgC12 for 12 min, followed by 75% alcohol for 15 s. The suitable cuhure medium for callus induction and differentiation was MS. The optimum medium for subculture multiplication was MS＋0.5 mg/L6-BA＋0.5 mg/L NAA, and the muhiplication coefficients of Tiber, Sorbonne and Siberi were 3.32, 3.32 and 3.00, respectively. The optimum culture
ISSN:2095-1191
DOI:10.3969/j:issn.2095-1191.2016.06.889