一种马铃薯块茎蛾卵的计数新方法

为了解决常规马铃薯块茎蛾卵计数过程中存在的工作量大、卵聚集程度高时计数不准确等,建立了一种新的卵粒计数方法,即采用70%乙醇浸泡卵30 min,用毛笔轻轻刷下,再置于显微镜下计数。经过方法学考察,洗脱计数法的准确性高、精密度、重复性好、操作简便,可用于马铃薯块茎蛾卵粒的计数。...

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Bibliographic Details
Published in江西农业学报 Vol. 29; no. 6; pp. 63 - 65
Main Author 吴梅 刘燕 李庭喜 段平 肖春
Format Journal Article
LanguageChinese
Published 云南农业大学植物保护学院,云南昆明650201 2017
云南中医学院中药学院,云南昆明650500%云南农业大学植物保护学院,云南昆明,650201%云南中医学院中药学院,云南昆明,650500
Subjects
卵粒
计数方法
马铃薯块茎蛾
计数方法
Counting method
Potato tuber moth
Egg
马铃薯块茎蛾
卵粒
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ISSN1001-8581
DOI10.19386/j.cnki.jxnyxb.2017.06.14

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Summary:为了解决常规马铃薯块茎蛾卵计数过程中存在的工作量大、卵聚集程度高时计数不准确等,建立了一种新的卵粒计数方法,即采用70%乙醇浸泡卵30 min,用毛笔轻轻刷下,再置于显微镜下计数。经过方法学考察,洗脱计数法的准确性高、精密度、重复性好、操作简便,可用于马铃薯块茎蛾卵粒的计数。
Bibliography:36-1124/S
In order to overcome the shortcomings of the conventional method for the counting of eggs of potato tuber moth, such as large workload, inaccurate counting results due to the high gathering of eggs, this research established a new method for the counting of eggs of potato tuber moth ： soaking eggs in 70% ethanol solution for half an hour, then brushing the eggs down gen- tly by brush pen, finally counting them under a microscope. The methodological survey proves that this method is accurate, pre- cise, repeatable and simple, and it can be applied to count the eggs of potato tuber moth.
Potato tuber moth; Egg; Counting method
WU Mei1,2, LIU Yan1, LI Ting-xi2, DUAN Ping2, XIAO Chun1. （ 1. College of Plant Protection, Yunnan Agricultural University, Kunming 650201, China; 2. College of Pharmaceutical Science, Yunnan University of Traditional Chinese Medicine, Kunming 650500, China）
ISSN:1001-8581
DOI:10.19386/j.cnki.jxnyxb.2017.06.14