巨大型蒲公英小分子量糖蛋白的制备及抗氧化活性分析

【目的】研究巨大型蒲公英小分子量糖蛋白的单糖组成及其抗氧化活性,为巨大型蒲公英资源的开发利用提供理论依据。【方法】以巨大型蒲公英为材料,通过水提醇沉法提取蒲公英粗糖蛋白,经Sephadex G-100柱、DEAE-52柱和透析法分离纯化得到蒲公英小分子量糖蛋白,用GC分析其单糖组成;同时采用磷钼络合物法、Fenton氧化法和邻苯三酚自氧化—分光光度法测定蒲公英小分子量糖蛋白的总抗氧化活性及其对羟基自由基（·OH）和超氧阴离子自由基（）的清除能力。【结果】巨大型蒲公英小分子量糖蛋白的多糖与蛋白质比例为1.722∶1,小分子量糖蛋白的单糖组成成分有鼠李糖、甘聚糖和葡萄糖。巨大型蒲公英小分子量糖蛋白...

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Bibliographic Details
Published in南方农业学报 Vol. 46; no. 3; pp. 486 - 491
Main Author 范勇 李宝宝 毛苇 梁引库
Format Journal Article
LanguageChinese
Published 陕西理工学院生物科学与工程学院,陕西汉中,723000 2015
Subjects
分离纯化
单糖组成
小分子量糖蛋白
抗氧化活性
蒲公英
单糖组成
monosaccharide composition
小分子量糖蛋白
蒲公英
抗氧化活性
分离纯化
isolation & purification
antioxidant activity
dandelion
low molecular weight glycoprotein
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ISSN2095-1191
DOI10.3969/j:issn.2095-1191.2015.3.486

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Summary:【目的】研究巨大型蒲公英小分子量糖蛋白的单糖组成及其抗氧化活性,为巨大型蒲公英资源的开发利用提供理论依据。【方法】以巨大型蒲公英为材料,通过水提醇沉法提取蒲公英粗糖蛋白,经Sephadex G-100柱、DEAE-52柱和透析法分离纯化得到蒲公英小分子量糖蛋白,用GC分析其单糖组成;同时采用磷钼络合物法、Fenton氧化法和邻苯三酚自氧化—分光光度法测定蒲公英小分子量糖蛋白的总抗氧化活性及其对羟基自由基（·OH）和超氧阴离子自由基（）的清除能力。【结果】巨大型蒲公英小分子量糖蛋白的多糖与蛋白质比例为1.722∶1,小分子量糖蛋白的单糖组成成分有鼠李糖、甘聚糖和葡萄糖。巨大型蒲公英小分子量糖蛋白的总抗氧化活性及其对·OH和的清除率均随样品浓度的增加而增大,其中对·OH和的清除作用强于Vc,而总抗氧化活性弱于Vc。【结论】巨大型蒲公英小分子量糖蛋白具有良好的抗氧化活性,可作为天然食品抗氧化剂资源进行利用。
Bibliography:45-1381/S
[Objective]The monosaccharide composition and antioxidant activity of low molecular weight glycoprotein for giant dandelion were studied to provide theoretical references for the development and utilization of giant dande-lion resources. 【Method 】The dandelion crude glycoprotein was extracted from giant dandelion by water extraction and alcohol precipitation,and the low molecular weight giant dandelion glycoprotein was obtained by Sephadex G-100 column, DEAE-52 column and dialysis purifying. The single sugar composition was analyzed by GC, while phosphorus molybdenum complex method was used to measure the total antioxidant activity of low molecular weight giant dandelion glycoprotein and phosphomolybdenum complex assay, Fenton oxidation and pyrogallol autoxidation method were used to determine the low molecular weight giant dandelion glycoprotein on the hydroxyl radical（·OH） and superoxide anion radicals（） scavenging. 【Result】The results showed that the proportion of protein and polysaccharide was 1.
ISSN:2095-1191
DOI:10.3969/j:issn.2095-1191.2015.3.486