盐胁迫下沙枣生物固氮能力及氮素分配研究
[目的]应用^15N自然丰度法研究盐胁迫条件下沙枣的生物固氮能力，并探讨几个耐盐树种的氮素分配情况。[方法]通过耐盐树种的室内砂培试验，确定盐渍生境条件下以空气氮为唯一氮源的沙枣^15N相对丰度B值及其适宜的参比植物；估测室内和野外盐渍生境中沙枣的生物固氮能力；并通过测定碳、氮、磷元素含量探讨了几种耐盐树种的氮素分配情况。[结果]室内盐胁迫砂培试验条件下，沙枣的B值为-1.41‰；柽柳和白蜡可作为沙枣的参比植物；沙枣的生物固氮百分率为55.03%。野外中度盐渍环境中，沙枣生物固氮百分率为69.69%。试验条件下，沙枣、柽柳和白蜡3个耐盐树种中，沙枣植株及其叶、茎、根含氮量最高，且与其它树种差异...
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| Published in | 林业科学研究 Vol. 30; no. 6; pp. 985 - 992 |
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| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
北京市林业工作总站,北京100029
2017
国家林业局盐碱地研究中心,北京100091%国家林业局盐碱地研究中心,北京,100091 |
| Subjects | |
| Online Access | Get full text |
| ISSN | 1001-1498 |
| DOI | 10.13275/j.cnki.lykxyj.2017.06.014 |
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| Summary: | [目的]应用^15N自然丰度法研究盐胁迫条件下沙枣的生物固氮能力，并探讨几个耐盐树种的氮素分配情况。[方法]通过耐盐树种的室内砂培试验，确定盐渍生境条件下以空气氮为唯一氮源的沙枣^15N相对丰度B值及其适宜的参比植物；估测室内和野外盐渍生境中沙枣的生物固氮能力；并通过测定碳、氮、磷元素含量探讨了几种耐盐树种的氮素分配情况。[结果]室内盐胁迫砂培试验条件下，沙枣的B值为-1.41‰；柽柳和白蜡可作为沙枣的参比植物；沙枣的生物固氮百分率为55.03%。野外中度盐渍环境中，沙枣生物固氮百分率为69.69%。试验条件下，沙枣、柽柳和白蜡3个耐盐树种中，沙枣植株及其叶、茎、根含氮量最高，且与其它树种差异显著（P〈0.05）；沙枣植株氮/碳含量比以及氮/磷含量比最高；沙枣叶、茎和根的氮/磷含量比最高。野外中度盐渍环境中，沙枣叶的氮/碳含量比以及氮/磷含量比均为最高。[结论]^15N自然丰度法可用于研究盐胁迫条件下沙枣的生物固氮能力，柽柳和白蜡可作为参比植物；沙枣在盐胁迫下的固氮能力较强，是可应用于盐渍土生物改良的优良树种。本文为盐胁迫条件下利用^15N自然丰度法研究生物固氮作用提供方法参考，对于盐碱地生物治理中植物材料的选择和耐盐树种资源的综合开发利用具有重要意义。 |
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| Bibliography: | [Objective] To estimate the biological nitrogen fixation of Elaeagnus angustifolia under salt stress and to study the nitrogen distributions of several saline-tolerant trees species. [Method] Saline-tolerant trees were cultivated in greenhouse, and the value of ^15N natural abundance （B） and appropriate reference plants in saline habitat were determined; the biological nitrogen fixation abilities of E. angustifolia in greenhouse and field saline habitat were investigated; and the nitrogen distributions of several saline-tolerant trees were obtained by determination of carbon, nitrogen and phosphorus contents. [Result] The results of planting trials showed that the B value of E. angustifolia was -1.41‰; Tamarix chinensis or Fraxinus chinensis could be the reference plant of E. angustifolia; the biological nitrogen fixation percentage of E. angustifolia was 55.03%. The biological nitrogen fixation percentage of E. angustifolia in moderate salinity field was 69.69%. In the experimental condition, among three sal |
| ISSN: | 1001-1498 |
| DOI: | 10.13275/j.cnki.lykxyj.2017.06.014 |