灵芝多糖肽GL-PPS的结构研究

灵芝是我国传统大型药用真菌之一,属无隔担子菌亚纲,无褶菌目,灵芝科,灵芝属,常见的有赤芝[Ganoderma lucidum（Curtis：Fr.）P.karst]、紫芝[Ganoderma sinense（Zhao,Xu et Zhang）][1]。从赤芝[Ganoderma lucidum（Curtis：Fr.）P.karst]多糖肽中分离纯化具有活性成分GL-PPS,对其进行结构分析。经分离纯化得GL-PPS,通过高效液相凝胶渗透色谱法（high-performance gel-permeation chromatography,HPGPC）、气相色谱、甲基化分析和Smith降解等方法研...

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Bibliographic Details
Published in西南农业学报 Vol. 28; no. 2; pp. 793 - 796
Main Author 王赛贞 林占熺 林冬梅 林树钱 林志彬 李晶
Format Journal Article
LanguageChinese
Published 福州绿谷生物药业技术研究所,福建福州350002%国家菌草工程技术研究中心,福建福州,350002%北京大学医学部药理系,北京,100083 2015
国家菌草工程技术研究中心,福建福州350002
Subjects
GL-PPS
结构
赤芝
赤芝
Ganoderma lucidum
Structure
结构
GL-PPS
Online AccessGet full text
ISSN1001-4829
DOI10.16213/j.cnki.scjas.2015.02.064

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Summary:灵芝是我国传统大型药用真菌之一,属无隔担子菌亚纲,无褶菌目,灵芝科,灵芝属,常见的有赤芝[Ganoderma lucidum（Curtis：Fr.）P.karst]、紫芝[Ganoderma sinense（Zhao,Xu et Zhang）][1]。从赤芝[Ganoderma lucidum（Curtis：Fr.）P.karst]多糖肽中分离纯化具有活性成分GL-PPS,对其进行结构分析。经分离纯化得GL-PPS,通过高效液相凝胶渗透色谱法（high-performance gel-permeation chromatography,HPGPC）、气相色谱、甲基化分析和Smith降解等方法研究分析GL-PPS的结构。结果表明,GL-PPS的单糖组成为鼠李糖（Rha）、甘露糖（Man）和葡萄糖（Glc）,物质的量比为14.9∶9.7∶75.4,其Mw为3.4×104,Mn为2.6×104;Mw/Mn为1.30。Mp为3.8×104。GL-PPS的主链由1,3-与1,4-两者交替连接的葡萄糖构成,在部分主链葡萄糖的6位有分支,非还原末端均由葡萄糖构成。
Bibliography:Ganoderma lucidum GL-PPS Structure
A water-soluble polysaccharide peptide designated GL-PPS,was isolated and purified from the heteropolysaccharide of Ganoderma lucidum fruit body. Using a range of techniques,such as high-performance gel-permeation chromatography,HPGPC gas chromatography,methylation analysis and Smith degradation methods,the sample was shown to be polysaccharide peptide,mainly composed of rhamnose,mannose and glucose with the amount of substance ratio 14. 9∶ 9. 7∶ 75. 4 and the weight-average molecular weight of 3. 4 × 104,number-average molecular weight of 2. 6 × 104. GL-PP was most branched of 1,3-and 1,4-alternate connection of glucose,whereas GL-PPS was mainly six branches glucose,a reduction at the end which was composed of glucose.
51-1213/S
WANG Sai-zhen ,LIN Zhan-xi , LIN Dong-mei ,LIN Shu-qian ,LIN Zhi-bin ,LI Jing （ 1. China National Engineering Research Center of JUNCAO Technology, Fujian Fuzhou 350002, China; 2. Fuzhou Green Valley Biopharm Aceutical Research Institute, Fujian Fuzhou
ISSN:1001-4829
DOI:10.16213/j.cnki.scjas.2015.02.064