复方汉防己颗粒中野黄芩苷的定性鉴别和含量测定

目的 建立对复方汉防己颗粒中来源于半枝莲的有效成分野黄芩苷的定性鉴别和含量测定方法 。方法 采用薄层色谱法对野黄芩苷进行定性鉴别,采用高效液相色谱法进行含量测定。结果 薄层色谱图斑点显色清晰,阴性对照无干扰;含量测定野黄芩苷在22.4～156.8μg/ml质量浓度范围内与峰面积呈良好线性关系,r=0.999 6（n=5）,平均加样回收率为97.79%,RSD=0.32%（n=6）。结论 所建立方法 专属性强、重现性好,可作为复方汉防己颗粒的质量控制方法 。...

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Published in药学实践杂志 Vol. 34; no. 3; pp. 245 - 248
Main Author 张淑瑜 徐建江 于燕莉 毕云生 赵文静 刘倩 刘同琪
Format Journal Article
LanguageChinese
Published 济南军区总医院药剂科 ,山东济南,250031 2016
Subjects
半枝莲
复方汉防己颗粒
薄层色谱法
野黄芩苷
高效液相色谱法
compound granule tetrandra
sculellaria barbata
高效液相色谱法
半枝莲
TLC
复方汉防己颗粒
薄层色谱法
scutellarin
HPLC
野黄芩苷
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ISSN1006-0111
DOI10.3969/j.issn.1006-0111.2016.03.013

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Summary:目的 建立对复方汉防己颗粒中来源于半枝莲的有效成分野黄芩苷的定性鉴别和含量测定方法 。方法 采用薄层色谱法对野黄芩苷进行定性鉴别,采用高效液相色谱法进行含量测定。结果 薄层色谱图斑点显色清晰,阴性对照无干扰;含量测定野黄芩苷在22.4～156.8μg/ml质量浓度范围内与峰面积呈良好线性关系,r=0.999 6（n=5）,平均加样回收率为97.79%,RSD=0.32%（n=6）。结论 所建立方法 专属性强、重现性好,可作为复方汉防己颗粒的质量控制方法 。
Bibliography:31-1685/R
compound granule tetrandra ; sculellaria barbata; scutellarin; TLC; HPLC
ZHANG Shuyu, XU Jianjiang, YU Yanli, BI Yunsheng,ZHAO Wenjing, LIU Qian, LIU Tongqi （Department of Pharmacy, Ji- nan Military General Hospital, Jinan 250031, China）
Objective To develop the methods for identification and determination of seutellarin in compound Granule Tetrandra. Methods A TLC method was used to identify quality for scutellarin, and a HPLC method was used to determine the content of scutellarin. Results The spot was clear in TLC identification without interference of negative control. The sam- ple size of scutellarin had a good linear relationship with peak area r=0. 999 6（n=5）when ranged from 22.4-156.8 μg/ml, with the average recovery rate 97.79 % （ RSD=0.320%, n= 6）. Conclusion The method is simple, with good specificity and reproducibility, which can be used as the quality control for this preparation.
ISSN:1006-0111
DOI:10.3969/j.issn.1006-0111.2016.03.013