三黄连合剂对罗非鱼人工感染海豚链球菌病的预防效果
【目的】探讨三黄连合剂对由海豚链球菌感染罗非鱼发病的预防效果,为罗非鱼链球菌病的免疫防治提供参考依据。【方法】分别以含30、60、120 m L/kg三黄连合剂的饵料投喂吉富罗非鱼,连续投喂7 d,给药后第8 d进行人工感染海豚链球菌,感染后第7 d测定脾脏指数,同时于感染后0、12、24 h和7 d采集血清样本,检测超氧化物歧化酶(SOD)、溶菌酶(LSZ)、碱性磷酸酶(AKP)、血清补体C3、Ig M抗体水平等免疫学指标。【结果】三黄连合剂连续给药7 d对吉富罗非鱼血清免疫指标均有一定影响,可显著提高吉富罗非鱼血清SOD活性(P〈0.05,下同)。人工感染海豚链球菌后第7 d,3个三黄连合...
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          | Published in | 南方农业学报 Vol. 46; no. 5; pp. 911 - 916 | 
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| Main Author | |
| Format | Journal Article | 
| Language | Chinese | 
| Published | 
            广西大学动物科学技术学院,南宁530005%广西大学动物科学技术学院,南宁,530005%广西水产科学研究院/广西水产遗传育种与健康养殖重点实验室,南宁,530021
    
        2015
     广西水产科学研究院/广西水产遗传育种与健康养殖重点实验室,南宁530021  | 
| Subjects | |
| Online Access | Get full text | 
| ISSN | 2095-1191 | 
| DOI | 10.3969/j:issn.2095-1191.2015.5.911 | 
Cover
| Summary: | 【目的】探讨三黄连合剂对由海豚链球菌感染罗非鱼发病的预防效果,为罗非鱼链球菌病的免疫防治提供参考依据。【方法】分别以含30、60、120 m L/kg三黄连合剂的饵料投喂吉富罗非鱼,连续投喂7 d,给药后第8 d进行人工感染海豚链球菌,感染后第7 d测定脾脏指数,同时于感染后0、12、24 h和7 d采集血清样本,检测超氧化物歧化酶(SOD)、溶菌酶(LSZ)、碱性磷酸酶(AKP)、血清补体C3、Ig M抗体水平等免疫学指标。【结果】三黄连合剂连续给药7 d对吉富罗非鱼血清免疫指标均有一定影响,可显著提高吉富罗非鱼血清SOD活性(P〈0.05,下同)。人工感染海豚链球菌后第7 d,3个三黄连合剂处理组的罗非鱼脾脏指数较感染不给药组明显升高;在免疫指标方面,高剂量组(120m L/kg)罗非鱼的血清SOD活性、AKP活性、LSZ活性、补体C3活性、Ig M抗体水平均显著或极显著(P〈0.01)高于感染不给药组。【结论】三黄连合剂可减轻因海豚链球菌感染而造成的免疫抑制,保护机体免疫系统,提升免疫力,对罗非鱼感染海豚链球菌有良好的预防效果。 | 
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| Bibliography: | 45-1381/S [Objective]The preventive effect of Chinese herbal compound "Sanhuanglian" on tilapia streptococcicosis was investigated to provide theoretical references for immunnologieal control of tilapia streptoeoccicosis. [Method]Three groups of tilapia were feeded with 30, 60 and 120 mL/kg "Sanhuanglian" for 7 days, respectively, Tilapia from different groups were infected artificially with Dolphin Streptococcus on the 8th day after administration. On the 7~ day after infec- tion,the spleen index was determined. Meawhile the serum samples were collected at 0 h, 12 h,24 h and 7 d after infec- tion, respectively. Then the immunological indexes were determined, including the superoxide dismutase (SOD), lysozyme (LSZ), alkaline phosphatase (AKP), complement C3 and IgM antibody levels in serum. [Results]Feeding with Chinese herbal compound "Sanhuanglian" for 7 days had a positive impact on immunological indexes of tilapia as follows: the superoxide dismutase (SOD) activity in tilapia significantly increased(P〈0.05  | 
| ISSN: | 2095-1191 | 
| DOI: | 10.3969/j:issn.2095-1191.2015.5.911 |