光合作用合成相关差异蛋白对应基因在高油酸油菜近等基因系中表达规律研究

【目的】本文探索了光合作用相关基因表达与蛋白表达之间的关系，加速高油酸油菜育种进程。【方法】以高油酸油菜近等基因系出苗后7d、5～6叶期和越冬期的叶片、花瓣和授粉后20～35d的种子及角果皮为材料，利用定量PCR方法研究9个iTRAQ分析得到的差异蚕白对应基因在不同生育期表达规律。【结果】在出苗后7d、花期和授粉后20—35d，高油酸油菜的光合作用能力低于低油酸油菜；5～6叶期、越冬期高油酸油菜的光合作用能力高于低油酸油菜。Gil515616基因在高油酸油菜出苗后7d去根材料及低油酸油菜5～6叶期、越冬期叶片和授粉后20～35d角果皮中不表达，在花、授粉后20～35d种子中均不表达。giI29...

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Published in西南农业学报 Vol. 30; no. 7; pp. 1483 - 1487
Main Author 王晓丹 肖钢 张振乾 官春云 陈社员 邬贤梦 张诗远
Format Journal Article
LanguageChinese
Published 湖南农业大学农学院/南方粮油作物协同创新中心,湖南长沙,410128 2017
Subjects
光合作用
实时荧光定量PCR
高油酸油菜
Real-time quantitative PCR
高油酸油菜
High oleic acid rapeseed
光合作用
Photosynthesis
实时荧光定量PCR
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ISSN1001-4829
DOI10.16213/j.cnki.scjas.2017.7.003

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Summary:【目的】本文探索了光合作用相关基因表达与蛋白表达之间的关系，加速高油酸油菜育种进程。【方法】以高油酸油菜近等基因系出苗后7d、5～6叶期和越冬期的叶片、花瓣和授粉后20～35d的种子及角果皮为材料，利用定量PCR方法研究9个iTRAQ分析得到的差异蚕白对应基因在不同生育期表达规律。【结果】在出苗后7d、花期和授粉后20—35d，高油酸油菜的光合作用能力低于低油酸油菜；5～6叶期、越冬期高油酸油菜的光合作用能力高于低油酸油菜。Gil515616基因在高油酸油菜出苗后7d去根材料及低油酸油菜5～6叶期、越冬期叶片和授粉后20～35d角果皮中不表达，在花、授粉后20～35d种子中均不表达。giI297853098，gil297825149和gil312282567基因及sil50313237和gil79475768基因分别具有相同的表达规律。Gil297825149基因在高油酸油菜角果皮中是唯一上调表达基因。Gil312282897基因在高油酸油菜出苗后7d去根材料为唯一上调表达基因。【结论】这些基因可作为高油酸油菜育种材料筛选的参考基因，加快育种进程。
Bibliography:Real-time quantitative PCR ; Photosynthesis ; High oleic acid rapeseed
WANG Xiao-dan, XIAO Gang, ZHANG Zhen-qian , GUAN Chun-yun, CHEN She-yuan, WU Xian-meng, ZHANG Shi-yuan （ College of Agriculture, Hunan Agricultural University/Southern Regional Collaborative Innovation Center for Grain and Oil Crops in China Hunan Changsha 410128, China）
51-1213/S
[ Objective] In this paper, the relationship between gene expression and protein expression in photosynthesis were discovered so that the process of high oleic acid rape breeding could be accelerated. [ Method] The 7 days after emergence, 5 - 6 leaf stage, leaves of win- tering period, petals, seeds and pods skins 20 - 35 days after pollination of high oleie acid rapeseed isogenic lines were used as tested mate- rials to find out the expression rules in different development periods. The nine corresponding genes of the differential proteins which derived from the iTRAQ analysis were studied by Real-time quantitative PCR. [ Result] In the development of 7 days after
ISSN:1001-4829
DOI:10.16213/j.cnki.scjas.2017.7.003