漾濞大泡与云新高原核桃花粉活力及柱头可授性比较

【目的】明确漾濞大泡和云新高原2个核桃品种的花粉活力和柱头可授性。【方法】用氯化三苯基四氮唑（TTC）染色法测定花粉在3种贮藏条件下（室温、冰箱冷藏和冷冻）的活力变化;采用联苯胺-过氧化氢法、过氧化物酶-酯酶试纸法、苏丹红染色法3种方法测定不同时期雌花柱头可授性。【结果】在相同保存温度下,云新高原核桃的花粉活力高于漾濞大泡。冰箱冷藏条件有利于花粉保存,保存至第7天时漾濞大泡和云新高原的花粉活力分别从第1天的88.7%和95.5%下降至10.0%和18.3%。氧化物酶-酯酶试纸法检测柱头可授性操作简单、效果较好。【结论】漾濞大泡和云新高原核桃的雌花柱头在V字期开始具有一定的可授性,八字期具有较强...

Full description

Saved in:
Bibliographic Details
Published in西南农业学报 Vol. 30; no. 6; pp. 1434 - 1438
Main Author 胡青 肖良俊 吴涛 宁德鲁 陈少瑜
Format Journal Article
LanguageChinese
Published 云南省林业科学院,云南昆明650201 2017
云南省木本油料工程技术研究中心,云南昆明650201
Subjects
云新高原
柱头可授性
深纹核桃
漾濞大泡
花粉活力
深纹核桃
云新高原
花粉活力
Juglans sigillata
柱头可授性
Stigma receptivity
漾濞大泡
Yangbidapao
Yunxingaoyuan
Pollen viability
Online AccessGet full text
ISSN1001-4829
DOI10.16213/j.cnki.scjas.2017.6.033

Cover

More Information
Summary:【目的】明确漾濞大泡和云新高原2个核桃品种的花粉活力和柱头可授性。【方法】用氯化三苯基四氮唑（TTC）染色法测定花粉在3种贮藏条件下（室温、冰箱冷藏和冷冻）的活力变化;采用联苯胺-过氧化氢法、过氧化物酶-酯酶试纸法、苏丹红染色法3种方法测定不同时期雌花柱头可授性。【结果】在相同保存温度下,云新高原核桃的花粉活力高于漾濞大泡。冰箱冷藏条件有利于花粉保存,保存至第7天时漾濞大泡和云新高原的花粉活力分别从第1天的88.7%和95.5%下降至10.0%和18.3%。氧化物酶-酯酶试纸法检测柱头可授性操作简单、效果较好。【结论】漾濞大泡和云新高原核桃的雌花柱头在V字期开始具有一定的可授性,八字期具有较强的可授性,是其授粉的最佳时期。
Bibliography:Juglans sigillata ; Ymagbidapao ; Yunxingaoyuan ; Pollen viability ; Stigma receptivity
[ Objective ] The pollen viability and stigma receptivity of two walnut cuhivars （ J. sigillata cv. ＇ Yangbidapao＇ and J. sigiUata regia cv. ＇ Yunxingaoyuan＇ ） planted in Kunming were studied in this paper. [ Method] The period and conditions （ room temperature, cold-storage and frozen-storage） for keeping high pollen viability were detected by triphenyltetrazolium chloride （TIC） staining method. The stig-ma receptivity and optimal pollination period were evaluated by benzidine-H2 02 , peroxidase-esterase reagent and sudan HI staining method.[ Result ] The results showed that the pollen viability of Yunxingaoyuan was higher than that of Yangbidapao at the same storage condition.The cold-storage was conducive to keeping high pollen viability, and which of Yangbidapao and Yunxingaoyuan were decreased to 10.0 %and 18.3 % respeotively after seven days. [ Conclusion] The peroxidase-esterase reagent method was the best way to mon
ISSN:1001-4829
DOI:10.16213/j.cnki.scjas.2017.6.033