81份烟草种质资源遗传多样性分析
从250对引物中筛选出64对SSR引物,分析了81份烟草种质资源的遗传多样性。结果表明：64对SSR引物在81份烟草种质材料中共扩增出269个基因位点,观测杂合度（Ho）平均值为0.3545,预期杂合度（He）的平均值为0.5425,Shannon’s信息指数（I=0.997）和Nei’s多样性指数（H=0.5391）较高,遗传分化系数（Fst=0.1454）较高,基因流（Nm=1.4699）较小,遗传相似系数较小（0.53～0.90）。说明81份烟草种质的遗传多样性较高,居群间的遗传分化水平较高,大部分位点表现出偏离Hardy-Weinberg平衡,杂合度不足,居群间有较小的基因流。...
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| Published in | 江西农业学报 Vol. 29; no. 1; pp. 62 - 68 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
河南农业大学 烟草学院,河南 郑州,450002
2017
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1001-8581 |
| DOI | 10.19386/j.cnki.jxnyxb.2017.01.13 |
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| Abstract | 从250对引物中筛选出64对SSR引物,分析了81份烟草种质资源的遗传多样性。结果表明：64对SSR引物在81份烟草种质材料中共扩增出269个基因位点,观测杂合度（Ho）平均值为0.3545,预期杂合度（He）的平均值为0.5425,Shannon’s信息指数（I=0.997）和Nei’s多样性指数（H=0.5391）较高,遗传分化系数（Fst=0.1454）较高,基因流（Nm=1.4699）较小,遗传相似系数较小（0.53～0.90）。说明81份烟草种质的遗传多样性较高,居群间的遗传分化水平较高,大部分位点表现出偏离Hardy-Weinberg平衡,杂合度不足,居群间有较小的基因流。 |
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| AbstractList | 从250对引物中筛选出64对SSR引物,分析了81份烟草种质资源的遗传多样性。结果表明：64对SSR引物在81份烟草种质材料中共扩增出269个基因位点,观测杂合度（Ho）平均值为0.3545,预期杂合度（He）的平均值为0.5425,Shannon’s信息指数（I=0.997）和Nei’s多样性指数（H=0.5391）较高,遗传分化系数（Fst=0.1454）较高,基因流（Nm=1.4699）较小,遗传相似系数较小（0.53～0.90）。说明81份烟草种质的遗传多样性较高,居群间的遗传分化水平较高,大部分位点表现出偏离Hardy-Weinberg平衡,杂合度不足,居群间有较小的基因流。 S572.024; 从250对引物中筛选出64对SSR引物，分析了81份烟草种质资源的遗传多样性。结果表明：64对SSR引物在81份烟草种质材料中共扩增出269个基因位点，观测杂合度（ Ho）平均值为0．3545，预期杂合度（ He）的平均值为0．5425，Shannon’ s信息指数（ I＝0．997）和Nei’ s多样性指数（ H＝0．5391）较高，遗传分化系数（ Fst＝0．1454）较高，基因流（Nm＝1．4699）较小，遗传相似系数较小（0．53～0．90）。说明81份烟草种质的遗传多样性较高，居群间的遗传分化水平较高，大部分位点表现出偏离Hardy－Weinberg平衡，杂合度不足，居群间有较小的基因流。 |
| Abstract_FL | The genetic diversities of 81 tobacco germplasm resources were analyzed by using 64 pairs of polymorphic SSR primers screened from 250 pairs of SSR primers.The results revealed that a total of 269 genetic loci were amplified in 81 tobacco materials through 64 pairs of polymorphic SSR primers.The average observed heterozygosity (Ho) was 0.3545 per primer pair, and the average expected heterozygosity ( He) was 0.5425 per primer pair.The Shannon’ s information index ( I=0.997) , Nei’ s diversity index ( H=0.5391) and genetic differentiation coefficient ( Fst=0 .1454)were relatively high , while the gene flow( Nm=1.4699) and genetic similarity coefficient (0.53~0.90) were relatively low.The above results indicated that the genetic diversi-ties of 81 tobacco germplasm resources were relatively high, the genetic differentiation level among different populations was rela-tively high, most loci seriously deviated from Hardy-Weinberg equilibrium, their heterozygosity was low,and the gene flow among different populations was less. |
| Author | 张铭真 李晓辉 王袁 杨铁钊 徐世晓 |
| AuthorAffiliation | 河南农业大学烟草学院,河南郑州450002 |
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| Author_FL | YANG Tie-zhao ZHANG Ming-zhen LI Xiao-hui XU Shi-xiao WANG Yuan |
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| ClassificationCodes | S572.024 |
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| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
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| DocumentTitleAlternate | Analysis of Genetic Diversity of 81 Tobacco Germplasm Resources |
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| EndPage | 68 |
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| Keywords | Tobacco Fluorescent SSR marker 烟草 遗传多样性 种质资源 荧光SSR Germplasm resources Genetic diversity |
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| Notes | ZHANG Ming-zhen, LI Xiao-hui, WANG Yuan, YANG Tie-zhao, XU Shi-xiao （ College of Tobacco Science, Henan Agricultural University, Zhengzhou 450002, China） 36-1124/S Tobacco; Germplasm resources; Fluorescent SSR marker; Genetic diversity The genetic diversities of 81 tobacco germplasm resources were analyzed by using 64 pairs of polymorphic SSR primers screened from 250 pairs of SSR primers. The results revealed that a total of 269 genetic loci were amplified in 81 tobacco materials through 64 pairs of polymorphic SSR primers. The average observed heterozygosity （Ho） was 0.3545 per primer pair, and the average expected heterozygosity （He） was 0.5425 per primer pair. The Shannon＇ s information index （I= 0.997） , Nei＇ s diversity index （H= 0.5391 ） and genetic differentiation coefficient （Fst = 0.1454） were relatively high, while the gene flow （Nm = 1.4699） and genetic similarity coefficient （0.55- 0.90） were relatively low. The above results indicated that the genetic diversities of 81 tobacco germplasm resources w |
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| PublicationTitle | 江西农业学报 |
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| Publisher | 河南农业大学 烟草学院,河南 郑州,450002 |
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| Snippet | 从250对引物中筛选出64对SSR引物,分析了81份烟草种质资源的遗传多样性。结果表明：64对SSR引物在81份烟草种质材料中共扩增出269个基因位点,观测杂合度（Ho）平均值... S572.024; 从250对引物中筛选出64对SSR引物，分析了81份烟草种质资源的遗传多样性。结果表明：64对SSR引物在81份烟草种质材料中共扩增出269个基因位点，观测杂合度（ Ho）... |
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| SubjectTerms | 烟草 种质资源 荧光SSR 遗传多样性 |
| Title | 81份烟草种质资源遗传多样性分析 |
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