81份烟草种质资源遗传多样性分析

从250对引物中筛选出64对SSR引物,分析了81份烟草种质资源的遗传多样性。结果表明：64对SSR引物在81份烟草种质材料中共扩增出269个基因位点,观测杂合度（Ho）平均值为0.3545,预期杂合度（He）的平均值为0.5425,Shannon’s信息指数（I=0.997）和Nei’s多样性指数（H=0.5391）较高,遗传分化系数（Fst=0.1454）较高,基因流（Nm=1.4699）较小,遗传相似系数较小（0.53～0.90）。说明81份烟草种质的遗传多样性较高,居群间的遗传分化水平较高,大部分位点表现出偏离Hardy-Weinberg平衡,杂合度不足,居群间有较小的基因流。...

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Bibliographic Details
Published in江西农业学报 Vol. 29; no. 1; pp. 62 - 68
Main Author 张铭真 李晓辉 王袁 杨铁钊 徐世晓
Format Journal Article
LanguageChinese
Published 河南农业大学 烟草学院,河南 郑州,450002 2017
Subjects
烟草
种质资源
荧光SSR
遗传多样性
Tobacco
Fluorescent SSR marker
烟草
遗传多样性
种质资源
荧光SSR
Germplasm resources
Genetic diversity
Online AccessGet full text
ISSN1001-8581
DOI10.19386/j.cnki.jxnyxb.2017.01.13

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Summary:从250对引物中筛选出64对SSR引物,分析了81份烟草种质资源的遗传多样性。结果表明：64对SSR引物在81份烟草种质材料中共扩增出269个基因位点,观测杂合度（Ho）平均值为0.3545,预期杂合度（He）的平均值为0.5425,Shannon’s信息指数（I=0.997）和Nei’s多样性指数（H=0.5391）较高,遗传分化系数（Fst=0.1454）较高,基因流（Nm=1.4699）较小,遗传相似系数较小（0.53～0.90）。说明81份烟草种质的遗传多样性较高,居群间的遗传分化水平较高,大部分位点表现出偏离Hardy-Weinberg平衡,杂合度不足,居群间有较小的基因流。
Bibliography:ZHANG Ming-zhen, LI Xiao-hui, WANG Yuan, YANG Tie-zhao, XU Shi-xiao （ College of Tobacco Science, Henan Agricultural University, Zhengzhou 450002, China）
36-1124/S
Tobacco; Germplasm resources; Fluorescent SSR marker; Genetic diversity
The genetic diversities of 81 tobacco germplasm resources were analyzed by using 64 pairs of polymorphic SSR primers screened from 250 pairs of SSR primers. The results revealed that a total of 269 genetic loci were amplified in 81 tobacco materials through 64 pairs of polymorphic SSR primers. The average observed heterozygosity （Ho） was 0.3545 per primer pair, and the average expected heterozygosity （He） was 0.5425 per primer pair. The Shannon＇ s information index （I= 0.997） , Nei＇ s diversity index （H= 0.5391 ） and genetic differentiation coefficient （Fst = 0.1454） were relatively high, while the gene flow （Nm = 1.4699） and genetic similarity coefficient （0.55- 0.90） were relatively low. The above results indicated that the genetic diversities of 81 tobacco germplasm resources w
ISSN:1001-8581
DOI:10.19386/j.cnki.jxnyxb.2017.01.13