聚石斛花粉生活力及贮藏的研究
以聚石斛花粉为试材，研究了其花粉生活力的检测方法及贮藏方法。结果表明：（1）检测聚石斛花粉萌发适宜的培养基为100g·L^-1蔗糖＋100mg·L^-1 H3803＋80mg·L^-1 CaCl2，TTC染色法不适用于聚石斛花粉生活力的检测；（2）聚石斛开花第0～8天的花粉生活力高于65％，作为父本，授粉结实率高于85％，显著高于蕾期和衰败期。（3）不同贮藏方法对聚石斛花粉生活力的影响较大，4℃冷藏干燥贮藏80d后，花粉生活力下降为0，而4℃冷藏湿润贮藏、-20℃冷冻湿润贮藏和干燥贮藏90d后，花粉生活力分别为1．4％、21．7％、6．4％。经-20℃冷冻湿润贮藏90d后的花粉经杂交授粉后仍然...
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| Published in | 林业科学研究 Vol. 27; no. 5; pp. 657 - 661 |
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| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
国家林业局林木培育重点实验室,中国林业科学研究院林业研究所,北京100091
2014
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1001-1498 |
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| Summary: | 以聚石斛花粉为试材，研究了其花粉生活力的检测方法及贮藏方法。结果表明：（1）检测聚石斛花粉萌发适宜的培养基为100g·L^-1蔗糖＋100mg·L^-1 H3803＋80mg·L^-1 CaCl2，TTC染色法不适用于聚石斛花粉生活力的检测；（2）聚石斛开花第0～8天的花粉生活力高于65％，作为父本，授粉结实率高于85％，显著高于蕾期和衰败期。（3）不同贮藏方法对聚石斛花粉生活力的影响较大，4℃冷藏干燥贮藏80d后，花粉生活力下降为0，而4℃冷藏湿润贮藏、-20℃冷冻湿润贮藏和干燥贮藏90d后，花粉生活力分别为1．4％、21．7％、6．4％。经-20℃冷冻湿润贮藏90d后的花粉经杂交授粉后仍然能够正常结实，其他处理的结实率明显降低。 |
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| Bibliography: | 11-1221/S DENG Xi-mei, ZHENG Bao-qiang, GUO Xin, WANG Yan （ Research Institute of Forestry, Chinese Academy of Forestry, Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration,Beijing 100091, China） The pollen of Dendrobium lindleyi was employed as experimental material to investigate the determination method of pollen viability and pollen storage. The results showed that ： （ 1 ） The optimum culture medium was 100 g·L^-1 sucrose ＋ 100 mg·L^-1 H3BO3 ＋ 80 mg · L^-1 CaC12. Trc staining was not adapted to detect pollen viabili- ty of Den. lindleyi. （2） The pollen germinating rate was over 65% during 0 -- 8 days after blooming. As pollen parents, the male receptivity was over 85% during this period, which showed a better result than bud stage and droop stage. （3） Different storage methods had different effects on pollen viability of Den. lindleyi. The viability of pollen stored at dry condition in 4 ℃ declined to 0 after 80 days. However, after stored at humid condition in 4 ℃, freeze-wetting |
| ISSN: | 1001-1498 |