鲮鱼源无乳链球菌的鉴定、血清型分析及药敏试验

【目的】确诊患病鲮鱼的发病原因,并对其病原菌进行分子血清型及药敏性分析,为有效防治该病提供参考依据,进而促进鲮鱼养殖业健康持续发展。【方法】以常规方法进行患病鲮鱼的病原菌分离纯化,通过形态观察、生理生化鉴定、16S rRNA基因序列分析及多位点序列分型（MLST分型）进行鉴定,并采用药敏纸片扩散法进行药敏试验。【结果】从患病鲮鱼体内分离获得1株优势菌株（GYCm081）,为呈链状排列的革兰氏阳性球菌,其16S rRNA基因片段与无乳链球菌16S rRNA序列（F0393392和NR_102871）的同源性最高,相似性均为100%,系统发育进化树也显示GYCm081菌株与无乳链球菌亲缘关系最近,...

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Bibliographic Details
Published in南方农业学报 Vol. 46; no. 11; pp. 2053 - 2058
Main Author 刘礼辉 张德锋 李宁求 石存斌 颜曦 付小哲 林强
Format Journal Article
LanguageChinese
Published 中国水产科学研究院珠江水产研究所/农业部渔用药物创制重点实验室/广东省水产动物免疫技术重点实验室,广州510380 2015
淡水水产养殖湖北省协同创新中心,武汉430070%中国水产科学研究院珠江水产研究所/农业部渔用药物创制重点实验室/广东省水产动物免疫技术重点实验室,广州,510380
Subjects
分离鉴定
无乳链球菌
药敏试验
血清型分析
鲮鱼
C.molitorella
serotype analysis
无乳链球菌
isolation and identification
血清型分析
S.agalactiae
药敏试验
drug sensitivity test
鲮鱼
分离鉴定
Online AccessGet full text
ISSN2095-1191
DOI10.3969/j:issn.2095-1191.2015.11.2053

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Summary:【目的】确诊患病鲮鱼的发病原因,并对其病原菌进行分子血清型及药敏性分析,为有效防治该病提供参考依据,进而促进鲮鱼养殖业健康持续发展。【方法】以常规方法进行患病鲮鱼的病原菌分离纯化,通过形态观察、生理生化鉴定、16S rRNA基因序列分析及多位点序列分型（MLST分型）进行鉴定,并采用药敏纸片扩散法进行药敏试验。【结果】从患病鲮鱼体内分离获得1株优势菌株（GYCm081）,为呈链状排列的革兰氏阳性球菌,其16S rRNA基因片段与无乳链球菌16S rRNA序列（F0393392和NR_102871）的同源性最高,相似性均为100%,系统发育进化树也显示GYCm081菌株与无乳链球菌亲缘关系最近,可确定为无乳链球菌。GYCm081菌株分子血清型为Ib型,MLST型为ST261型,对氨苄西林、氯霉素、壮观霉素、红霉素、恩诺沙星、氧氟沙星、利福平、环丙沙星、呱拉西林、新生霉素、头孢唑啉、四环素和呋喃妥因等13种药物高度敏感而对磺胺甲噁唑、磺胺甲异噁唑和青霉素G已经产生耐药性。【结论】引起广东阳江某鲮鱼养殖场发病死亡的病原菌为无乳链球菌,其分子血清型为Ib-ST261,实际生产中可选用氨苄西林、恩诺沙星和氧氟沙星等药物进行防治。
Bibliography:45-1381/S
C.molitorella; S.agalactiae; isolation and identification; serotype analysis; drug sensitivity test
LIU Li-hui, ZHANG De-feng , LI Ning-qiu, SHI Cun-bin, YAN Xi, FU Xiao-zhe, LIN Qiang （1Pearl River Fishery Research Institute, Chinese Academy of Fishery Sciences/Key Laboratory of Fishery Drug Development, Ministry of Agriculture/Key Laboratory of Aquatic Animal Immune Technology, Guangzhou 510380, China; 2Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan 430070, China）
[Objective]The present experiment was conducted to investigate pathogenesis of diseased Cirrhinus molitorella and analyze serotype and drug sensitivity of pathogen isolated from diseased C.molitorella,in order to provide reference for controling disease and promoting sustainable development of C.molitorella aquaculture industry.[Method]The pathogen was isolated from diseased C.molitorella.And the isolated pathogen was identified through morphologic observation,physiological and biochemical properties determi
ISSN:2095-1191
DOI:10.3969/j:issn.2095-1191.2015.11.2053