AtMET1基因克隆及化学诱导表达分析

DNA甲基化是植物基因组中普遍存在的一种的重要表观遗传学机制,对植物生长发育及进化起着重要的调节作用[1-2]。植物体DNA甲基化的建立和维持受多个基因的协同调控,其中MET1（DN-MT1-like METHYLTRANSFERASE 1 gene）是在植物中最早分离出来的甲基化相关基因,编码DNA甲基化转移酶1（MET1,Methytransferase1）,该酶主要负责保持CG位点的甲基化,通过DNA甲基化作用影响植物的基因组结构、发育和进化。...

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Published in林业科学研究 Vol. 29; no. 6; pp. 946 - 950
Main Author 梁立雄 常英英 高亚南 王颜波 张伟溪 苏晓华 张冰玉
Format Journal Article
LanguageChinese
Published 林木遗传育种国家重点实验室，国家林业局林木培育重点实验室，中国林业科学研究院林业研究所，北京 100091 2016
Subjects
17-β-雌二醇
pER8-MET1
化学诱导表达载体
瞬时表达
transient expression
17-β-雌二醇
17-β-estradiol
chemical-inducible expression vector
瞬时表达
pER8-MET1
化学诱导表达载体
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ISSN1001-1498

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Summary:DNA甲基化是植物基因组中普遍存在的一种的重要表观遗传学机制,对植物生长发育及进化起着重要的调节作用[1-2]。植物体DNA甲基化的建立和维持受多个基因的协同调控,其中MET1（DN-MT1-like METHYLTRANSFERASE 1 gene）是在植物中最早分离出来的甲基化相关基因,编码DNA甲基化转移酶1（MET1,Methytransferase1）,该酶主要负责保持CG位点的甲基化,通过DNA甲基化作用影响植物的基因组结构、发育和进化。
Bibliography:11-1221/S
[Objective]In order to study the function of Arabidopsis thaliana methyltransferase gene At MET1,17-β-estradiol inducible plant expression vector p ER8-MET1 was constructed,and its characters of inducible expression were detected. [Methods]The At MET1 gene from Arabidopsis thaliana was used as the target gene,and‘digestionligation＇method was applied for constructing the plant expression vector p ER8-MET1,which could be induced by17-β-estradiol. Then the vector was transferred to Agrobacterium tumefaciens LBA4404. The Agrobacterium strain containing p ER8-GFP vector was injected into the leaves of Nicotiana tabacum,then the leaves were induced by 17-β-estradiol in different levels of concentrations and time durations. QPCR was performed to detect GFP gene expression,through which the optimum concentration and time duration were screened. The Agrobacterium strain containing p ER8-MET1 vector was injected into the leaves of Nicotiana tabacum,then the leaves were induced by optimal concentration of 17-β-
ISSN:1001-1498