圆叶葡萄叶提取物对南方根结线虫的生物活性

【目的】研究圆叶葡萄叶提取物对南方根结线虫的生物活性，为南方根结线虫防治提供一种高效、低毒的新途径。【方法】采用离体毒力测定研究圆叶葡萄叶乙醇提取物对南方根结线虫的杀虫活性；将圆叶葡萄叶乙醇提取物稀释成10．0、5．0、1．0g/L后处理已接种南方根结线虫的盆栽番茄，评估圆叶葡萄叶提取物对南方根结线虫的田间防治效果。【结果】圆叶葡萄叶乙醇提取物具有较强的毒杀线虫活性，浓度为16．7g／L时活性最强，处理48h线虫校正死亡率达99．06％，LC50为3．76g／L。经10．0、5．0、1．0g/L圆叶葡萄叶乙醇提取物处理后的番茄幼苗株高分别比对照提高30．35％、22．97％和5．38％，地上鲜...

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Published in南方农业学报 Vol. 46; no. 10; pp. 1812 - 1816
Main Author 李晓琼 唐军 毛润萍 黄羽 郭文锋
Format Journal Article
LanguageChinese
Published 广西大学林学院,南宁530005%广西农业科学院生物技术研究所,南宁,530007%广西作物遗传改良生物技术重点开放实验室,南宁,530007%广西农业科学院葡萄与葡萄酒研究所,南宁,530007 2015
广西作物遗传改良生物技术重点开放实验室,南宁530007
Subjects
乙醇提取物
南方根结线虫
圆叶葡萄
杀虫活性
muscadine (V.rotundifolia)
nematocidal activity
乙醇提取物
杀虫活性
ethanol extracts
圆叶葡萄
南方根结线虫
M.incognita
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ISSN2095-1191
DOI10.3969/j:issn.2095-1191.2015.10.1812

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Summary:【目的】研究圆叶葡萄叶提取物对南方根结线虫的生物活性，为南方根结线虫防治提供一种高效、低毒的新途径。【方法】采用离体毒力测定研究圆叶葡萄叶乙醇提取物对南方根结线虫的杀虫活性；将圆叶葡萄叶乙醇提取物稀释成10．0、5．0、1．0g/L后处理已接种南方根结线虫的盆栽番茄，评估圆叶葡萄叶提取物对南方根结线虫的田间防治效果。【结果】圆叶葡萄叶乙醇提取物具有较强的毒杀线虫活性，浓度为16．7g／L时活性最强，处理48h线虫校正死亡率达99．06％，LC50为3．76g／L。经10．0、5．0、1．0g/L圆叶葡萄叶乙醇提取物处理后的番茄幼苗株高分别比对照提高30．35％、22．97％和5．38％，地上鲜重分别比对照增加27．62％、19．33％和0．57％，根长分别比对照提高31．43％、27．69％和10．54％，植株根结抑制率分别达46．85％、40．29％和25．95％。【结论】圆叶葡萄叶提取物能减轻南方根结线虫危害、促进寄主植株生长，利用圆叶葡萄叶提取物防治南方根结线虫具有研发价值。
Bibliography:45-1381/S
LI Xiao-qiong , TANG Jun, MAO Run-ping, HUANG Yu, GUO Wen-feng （1Guangxi Crop Genetic Improvement and Biotechnology Lab, Nanning 530007, China; 2College of Forestry, Guangxi University, Nanning 530005, China; 3Biotechnology Research Institute, Guangxi Academy of Agricuhure Science, Nanning 530007, China; 4Viticuhure and Wine Research Institute, Guangxi Academy of Agricuhural Sciences, Nanning 530007, China）
muscadine（V, rotundifolia）; ethanol extracts; M. incognita; nematocidal activity
[Objective]In order to find a new effective but low toxic method for controlling southern root-knot nema- tode （Meloidogyne incognita）, the bioaetivities of muscadine （ Vitis rotundifolia） leaf extracts against M. incognita were in- vestigated. [ Method ]The nematoeidal activity of ethanol extracts from leaves of muscadine against M. incognita was deter- mined by in vitro bioassays. The ethanol extracts of muscadine leaves were diluted to 10.0, 5.0, 1.0 g/L, respectively. Then these diluted extracts were used to treat p
ISSN:2095-1191
DOI:10.3969/j:issn.2095-1191.2015.10.1812