基于液滴微流控的壳寡糖植物免疫诱导剂荧光检测

为了建立基于液滴微流控芯片平台的植物免疫诱导剂的荧光信号检测技术,该文设计制作了具有液滴生成结构的芯片,制备包裹烟草（BY-2）细胞的液滴,并对包裹了细胞的液滴数目进行统计分析。将包裹细胞的液滴孵育一氧化氮探针后使用质量浓度为50μg/m L壳寡糖处理,在荧光显微镜下观测其产生的荧光,利用荧光酶标仪对比采用96孔板和液滴承载的细胞的荧光变化趋势。结果显示,水相流速为100μL/h,油相流速为300μL/h时,微流控芯片产生的液滴尺寸适合包裹细胞,其中液滴包裹单细胞的比例达22.9%。经壳寡糖处理后的细胞在生成的液滴中产生了明显的荧光,且用96孔板和液滴承载的细胞的荧光变化趋势一致。研究结果为开...

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Bibliographic Details
Published in农业工程学报 Vol. 33; no. 2; pp. 302 - 307
Main Author 苍小鑫 赵小明 钟润涛 王文霞 尹恒 朱靖博 丁燕
Format Journal Article
LanguageChinese
Published 大连工业大学食品学院，大连 116034 2017
中国科学院大连化学物理研究所，大连 116023%中国科学院大连化学物理研究所,大连,116023%大连海事大学环境科学与工程学院,大连,116026%大连工业大学食品学院,大连,116034
Subjects
包裹
壳寡糖
微流控
植物免疫诱导剂
烟草细胞
荧光
微流控
BY-2 tobacco cell
壳寡糖
encapsulation
荧光
fluorescence
chitosan oligosaccharide
植物免疫诱导剂
烟草细胞
microfluidic
plant immune elicitors
包裹
Online AccessGet full text
ISSN1002-6819
DOI10.11975/j.issn.1002-6819.2017.02.042

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Summary:为了建立基于液滴微流控芯片平台的植物免疫诱导剂的荧光信号检测技术,该文设计制作了具有液滴生成结构的芯片,制备包裹烟草（BY-2）细胞的液滴,并对包裹了细胞的液滴数目进行统计分析。将包裹细胞的液滴孵育一氧化氮探针后使用质量浓度为50μg/m L壳寡糖处理,在荧光显微镜下观测其产生的荧光,利用荧光酶标仪对比采用96孔板和液滴承载的细胞的荧光变化趋势。结果显示,水相流速为100μL/h,油相流速为300μL/h时,微流控芯片产生的液滴尺寸适合包裹细胞,其中液滴包裹单细胞的比例达22.9%。经壳寡糖处理后的细胞在生成的液滴中产生了明显的荧光,且用96孔板和液滴承载的细胞的荧光变化趋势一致。研究结果为开发基于液滴微流控技术的植物免疫诱导剂的高通量筛选平台提供参考。
Bibliography:11-2047/S
Cang Xiaoxin1,2, Zhao Xiaoming2, Zhong Runtao3, Wang Wenxia2, Yin Heng2, Zhu Jingbo1, Ding Yan1＇~ （1. School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China; 2. Dalian Institute of Chemical Physics, Chinese Academic of Sciences, Dalian 116023, China; 3. School of Environmental Science and Engineering, Dalian Maritime University, Dalian 116026, China）
microfluidic; encapsulation; fluorescence; plant immune elicitors; BY-2 tobacco cell; chitosan oligosaccharide
The plant immune elicitors can induce a line of defense responses in plants and are identifed as new type of biological pesticides. At present stage, new plant immune elicitor is screening with potted plants or field experiments, which is time-consuming and high-cost. The droplet microfluidic technology, which is originated from analytical chemistry and owns micro-channel network structure, has properties of high throughput, high sensitivity, low consumption of reagent, no cross contamination and rapid reaction.
ISSN:1002-6819
DOI:10.11975/j.issn.1002-6819.2017.02.042