南方大口鲶烂尾病病原菌的分离鉴定及药敏试验

【目的】查明广西南宁邕江上游网箱养殖南方大口鲶烂尾病病原及其对药物的敏感性,为该病的有效防治提供参考。【方法】以常规方法进行病原菌分离与纯化,通过API 20E细菌鉴定系统和16S r RNA基因PCR扩增进行病原菌鉴定,并以K-B纸片扩散法进行药敏试验。【结果】从患病南方大口鲶病料中分离获得1株优势菌株（DKN01）,经鉴定为阪崎克罗诺杆菌（Cronobacter sakazakii）,与阪崎克罗诺杆菌标准菌株NBRC 102416和ATCC 29544的亲缘关系最近,同源性均达99.5%。菌株DKN01对南方大口鲶的致死率为70.0%～95.0%,对复达欣、菌必治、舒普深、多粘菌素B、左氟...

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Bibliographic Details
Published in南方农业学报 Vol. 46; no. 9; pp. 1720 - 1725
Main Author 黄伟德 黄艳华 梁静真 龙苏 牛志伟 范华龙 黄钧 曾桂忠
Format Journal Article
LanguageChinese
Published 广西水生动物病害诊断实验室,南宁530005 2015
钦州市水产技术推广站,广西钦州530021%广西水生动物病害诊断实验室,南宁530005
广西大学动物科学技术学院/广西高校水生生物健康养殖与营养调控重点实验室,南宁530005%武鸣县渔政监督管理站,广西武鸣,530100
梧州市水产技术推广站,广西梧州543002%广西水生动物病害诊断实验室,南宁530005
Subjects
分离鉴定
南方大口鲶
烂尾病
药敏试验
阪崎克罗诺杆菌
阪崎克罗诺杆菌
S.meridionalis
南方大口鲶
C.sakazakii
isolation and identification
烂尾病
药敏试验
drug sensitivity test
tail-rot disease
分离鉴定
Online AccessGet full text
ISSN2095-1191
DOI10.3969/j:issn.2095-1191.2015.09.1720

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Summary:【目的】查明广西南宁邕江上游网箱养殖南方大口鲶烂尾病病原及其对药物的敏感性,为该病的有效防治提供参考。【方法】以常规方法进行病原菌分离与纯化,通过API 20E细菌鉴定系统和16S r RNA基因PCR扩增进行病原菌鉴定,并以K-B纸片扩散法进行药敏试验。【结果】从患病南方大口鲶病料中分离获得1株优势菌株（DKN01）,经鉴定为阪崎克罗诺杆菌（Cronobacter sakazakii）,与阪崎克罗诺杆菌标准菌株NBRC 102416和ATCC 29544的亲缘关系最近,同源性均达99.5%。菌株DKN01对南方大口鲶的致死率为70.0%～95.0%,对复达欣、菌必治、舒普深、多粘菌素B、左氟沙星、先锋必、特治星、盐酸沙拉沙星等8种药物高度敏感,对青霉素G、复方新诺明、新生霉素、多西环素、氟苯尼考、磺胺间甲氧嘧啶、磺胺对甲氧嘧啶等7种药物则不敏感。【结论】引起广西南宁邕江上游网箱养殖南方大口鲶烂尾病的病原菌为阪崎克罗诺杆菌,生产中可选用复达欣、菌必治、舒普深、多粘菌素B、左氟沙星、先锋必、特治星和盐酸沙拉沙星等药物进行防治。
Bibliography:45-1381/S
S. meridionalis ; tail-rot disease; C. sakazakii; isolation and identification; drug sensitivity test
HUANG Wei-de, HUANG Yan-hua, LIANG Jing-zhen, LONG Su, NIU Zhi-wei, FAN Hua-long, HUANG Jun, ZENG Gui-zhong （1Guangxi Aquatic Animal Diseases Diagnosis Laboratory, Nanning 530005, China; 2Qinzhou Aquaculture Technology Extending Station, Qinzhou, Guangxi 530021, China; 3Wuzhou Aquaculture Technology Extending Stations, Wuzhou, Guangxi 543002, China; 4College of Animal Science and Technology, Guangxi University/Key Laboratory of Aquatic Health Cultivation and Nutrition Regulation of Guangxi Universities, Nanning 530005, China; 5Wuming Fishery Supervision and Management Station, Wuming, Guangxi 530100, China）
[Objective]In order to provided reference for effective prevention and control of tail-rot disease, the present experiment was conducted to investigate pathogen of tail-rot disease on Silurus meridionalis cultured with net cages in upstream of Yongjiang River in Nanning, Guangxi and drug sensitivity
ISSN:2095-1191
DOI:10.3969/j:issn.2095-1191.2015.09.1720