小麦黏型核质互作雄性不育—恢复近等基因系的构建及其遗传背景检测

【目的】构建小麦黏型核质互作雄性不育—恢复近等基因系,为进一步探索小麦雄性不育机理及对不育及恢复基因的精细定位和图位克隆提供参考。【方法】利用黏型小麦雄性不育系Ms（Kost）-5-1为母本与恢复系RK5451杂交获得F1代,以Ms（Kost）-5-1为轮回亲本与F1代及回交后代中的可育株连续回交6~7代构建黏型小麦核质互作雄性不育—恢复近等基因系;利用SSR标记、醇溶蛋白的A-PAGE与田间农艺性状相结合的方法对所构建的近等基因系进行检测。【结果】定向快速获得16个小麦黏型核质互作雄性不育—恢复近等基因系（N2~N17）。SSR分子标记及A-PAGE检测结果表明,各近等基因系遗传背景与恢复系...

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Bibliographic Details
Published in南方农业学报 Vol. 47; no. 8; pp. 1254 - 1260
Main Author 牛娜 马守才 王军卫 宋瑜龙 张改生
Format Journal Article
LanguageChinese
Published 西北农林科技大学农学院/教育部小麦育种工程研究中心,陕西杨凌,712100 2016
Subjects
分子标记
小麦
恢复基因
近等基因系
雄性不育
雄性不育
restorer gene
molecular marker
恢复基因
wheat
near-isogenic line
小麦
分子标记
male sterile
近等基因系
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ISSN2095-1191
DOI10.3969/j:issn.2095-1191.2016.08.1254

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Summary:【目的】构建小麦黏型核质互作雄性不育—恢复近等基因系,为进一步探索小麦雄性不育机理及对不育及恢复基因的精细定位和图位克隆提供参考。【方法】利用黏型小麦雄性不育系Ms（Kost）-5-1为母本与恢复系RK5451杂交获得F1代,以Ms（Kost）-5-1为轮回亲本与F1代及回交后代中的可育株连续回交6~7代构建黏型小麦核质互作雄性不育—恢复近等基因系;利用SSR标记、醇溶蛋白的A-PAGE与田间农艺性状相结合的方法对所构建的近等基因系进行检测。【结果】定向快速获得16个小麦黏型核质互作雄性不育—恢复近等基因系（N2~N17）。SSR分子标记及A-PAGE检测结果表明,各近等基因系遗传背景与恢复系RK5451的差异较明显,而与不育系Ms（Kost）-5-1遗传背景趋近相同,N4、N10、N11和N16保留了较好育性恢复性。农艺性状差异及聚类分析结果表明,各近等基因系仅在株高上呈显著（P〈0.05）或极显著（P〈0.01）差异,大多数农艺性状具有较高的相似性,N2、N3、N10、N16与不育系Ms（Kost）-5-1农艺性状差异最小。【结论】N10和N16为不育系Ms（Kost）-5-1较好的近等基因系,可用于进一步基因精细定位和图位克隆。
Bibliography:45-1381/S
wheat ; male sterile ; near-isogenic line ; restorer gene ; molecular marker
[ Objective ]The present experiment was conducted to construct nucleo-cytoplasmic male sterility-restorer near-isogenic lines（NILs） of wheat with Ae. kotschyi cytoplasm, in order to provide reference for further researching mechanism of male sterile, mapping restorer gene and mapping-based cloning. [ Method ]A male sterile line Ms（Kost）-5-1 was used as the recurrent parent to cross with restorer RK5451 and their Fj, and backcross with fertile plants of their off- spring for 6-7 generations to develop NILs of wheat with Ae. kotsehyi cytoplasm. The SSR molecular markers, polyacry- lamide gel electrophoresis （A-PAGE） methods combined with the agronomy traits were used to evaluate reliability of ob- tained NILs. [ Result ]A total of 16 nucleo-cytoplasmic male sterility-restorer NILs （N2-N 17） were obtained fast and accu- rately. The SSR molecular markers and A-PAGE showed that, the genetic background of all NILs were obviously di
ISSN:2095-1191
DOI:10.3969/j:issn.2095-1191.2016.08.1254