广西防城港市火龙果茎溃疡病病原鉴定

【目的】明确在广西防城港市发生的火龙果茎溃疡病病原菌种类,为更好地识别并控制该病害提供理论依据。【方法】从广西防城港市的火龙果园采集具有斑点、溃疡和腐烂症状的火龙果肉质茎,从中分离纯化病原菌,利用柯赫氏法则验证其致病性,通过形态学观察及分子生物学鉴定确定病原菌的种类。【结果】根据病原菌的形态学特征,通过对其ITS、β-tubulin和EF-1α3个基因序列的BLAST分析,将病原菌鉴定为新暗色柱节孢菌（Neoscytalidium dimidiatum）。【结论】广西防城港市火龙果茎溃疡病由新暗色柱节孢菌引起。...

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Bibliographic Details
Published in南方农业学报 Vol. 46; no. 9; pp. 1606 - 1612
Main Author 陆志翔 陆小平 秦斌华 成美华 黄林丹 陈保善 廖咏梅
Format Journal Article
LanguageChinese
Published 广西大学农学院,南宁,530005%广西水果生产技术指导总站,南宁,530022%东兴市农业技术推广中心,广西东兴,538100%亚热带农业生物资源保护与利用国家重点实验室,南宁,530005%广西大学农学院,南宁530005 2015
亚热带农业生物资源保护与利用国家重点实验室,南宁530005
Subjects
新暗色柱节孢菌
火龙果
茎溃疡病
防城港市
防城港市
pitaya
N.dimidiatum
火龙果
茎溃疡病
新暗色柱节孢菌
Fangchenggang city
stem canker disease
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ISSN2095-1191
DOI10.3969/j:issn.2095-1191.2015.09.1606

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Summary:【目的】明确在广西防城港市发生的火龙果茎溃疡病病原菌种类,为更好地识别并控制该病害提供理论依据。【方法】从广西防城港市的火龙果园采集具有斑点、溃疡和腐烂症状的火龙果肉质茎,从中分离纯化病原菌,利用柯赫氏法则验证其致病性,通过形态学观察及分子生物学鉴定确定病原菌的种类。【结果】根据病原菌的形态学特征,通过对其ITS、β-tubulin和EF-1α3个基因序列的BLAST分析,将病原菌鉴定为新暗色柱节孢菌（Neoscytalidium dimidiatum）。【结论】广西防城港市火龙果茎溃疡病由新暗色柱节孢菌引起。
Bibliography:45-1381/S
LU Zhi-xiang, LU Xiao-ping, QIN Bin-hua, CHENG Mei-hua, HUANG Lin-dan, CHEN Bao-shan, LIAO Yong-mei （1Agricultural College, Guangxi University, Nanning 530005, China; 2Guangxi Fruit Production Technology Extension Station, Nanning 530022, China; 3Dongxing Agro-tech Extension and Service Center, Dongxing, Guangxi 538100, China; 4State Key Laboratory for Conservation and Utilization Subtropical Agro-bioresources, Nanning 530005, China）
pitaya; stem canker disease; N. dimidiatum; Fangchenggang city
[Objective]In order to provide theoretical basis for recognizing and controling pitaya stem canker, the pathogenic species of pitaya stem canker disease occurred in Fangchenggang was identified. [Method]The fleshy stems of pitaya with spot, canker and rot symptoms were collected from pitaya orchard of Fangchenggang city, and the pathogenic fungi were isolated and purified. Then, the pathogenicity of pathogenic fungi was tested and verified according to the Koch＇s Rule, the pathogenic species was identified base
ISSN:2095-1191
DOI:10.3969/j:issn.2095-1191.2015.09.1606