我国几种重要松毛虫基因组大小测定
本研究采用流式细胞术，以公鸡（Callus domesticus）血红细胞DNA含量为标准，测定了我国南方几种重要松毛虫的基因组大小（或称C-值）。结果显示，马尾松毛虫（Dendrolimuspunctatus）的基因组大小为563．36±7.26Mb，其雌、雄2C DNA含量分别为1．1756±0．0164和1．1286±0．0134Pg；思茅松毛虫（Dendrolimuskikuchii）的基因组大小为719．30±9．70Mb，其雌、雄2C DNA含量分别为1．4492±0．0212和1．4428±O．0184pg；云南松毛虫（Den-drolimushoui）的基因组大小为586．82...
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| Published in | 林业科学研究 Vol. 27; no. 5; pp. 583 - 589 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
国家林业局森林保护学重点实验室,中国林业科学研究院森林生态环境与保护研究所,北京100091%广西壮族自治区森林病虫害防治站,广西南宁,530022%广西壮族自治区桂林市森林病虫害防治检疫站,广西桂林,541001
2014
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1001-1498 |
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| Abstract | 本研究采用流式细胞术，以公鸡（Callus domesticus）血红细胞DNA含量为标准，测定了我国南方几种重要松毛虫的基因组大小（或称C-值）。结果显示，马尾松毛虫（Dendrolimuspunctatus）的基因组大小为563．36±7.26Mb，其雌、雄2C DNA含量分别为1．1756±0．0164和1．1286±0．0134Pg；思茅松毛虫（Dendrolimuskikuchii）的基因组大小为719．30±9．70Mb，其雌、雄2C DNA含量分别为1．4492±0．0212和1．4428±O．0184pg；云南松毛虫（Den-drolimushoui）的基因组大小为586．82±8．94Mb，其雌、雄2CDNA含量分别为1．244±0．0182和1．156±O．0184Pg。统计分析结果发现，思茅松毛虫基因组显著大于云南松毛虫和马尾松毛虫基因组，后两者的基因组也存在显著差异，但绝对值相差较小。3种松毛虫雌雄虫的基因组大小之间也存在显著的差异，雌虫基因组大于雄虫。几种松毛虫基因组大小的测定，为进一步比较其分子差异、构建基因组文库和基因组学研究奠定了基础。 |
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| AbstractList | S763; 本研究采用流式细胞术,以公鸡(Gallus domesticus)血红细胞DNA含量为标准,测定了我国南方几种重要松毛虫的基因组大小(或称C-值).结果显示,马尾松毛虫(Dendrolimus punctatus)的基因组大小为563.36±7.26 Mb,其雌、雄2C DNA含量分别为1.175 6±0.016 4和1.128 6±0.013 4 pg;思茅松毛虫(Dendrolimus kikuchii)的基因组大小为719.30±9.70 Mb,其雌、雄2C DNA含量分别为1.449 2±0.021 2和1.442 8±0.018 4 pg;云南松毛虫(Den-drolimus houi)的基因组大小为586.82±8.94 Mb,其雌、雄2C DNA含量分别为1.244±0.0182和1.156±0.018 4 pg.统计分析结果发现,思茅松毛虫基因组显著大于云南松毛虫和马尾松毛虫基因组,后两者的基因组也存在显著差异,但绝对值相差较小.3种松毛虫雌雄虫的基因组大小之间也存在显著的差异,雌虫基因组大于雄虫.几种松毛虫基因组大小的测定,为进一步比较其分子差异、构建基因组文库和基因组学研究奠定了基础. 本研究采用流式细胞术，以公鸡（Callus domesticus）血红细胞DNA含量为标准，测定了我国南方几种重要松毛虫的基因组大小（或称C-值）。结果显示，马尾松毛虫（Dendrolimuspunctatus）的基因组大小为563．36±7.26Mb，其雌、雄2C DNA含量分别为1．1756±0．0164和1．1286±0．0134Pg；思茅松毛虫（Dendrolimuskikuchii）的基因组大小为719．30±9．70Mb，其雌、雄2C DNA含量分别为1．4492±0．0212和1．4428±O．0184pg；云南松毛虫（Den-drolimushoui）的基因组大小为586．82±8．94Mb，其雌、雄2CDNA含量分别为1．244±0．0182和1．156±O．0184Pg。统计分析结果发现，思茅松毛虫基因组显著大于云南松毛虫和马尾松毛虫基因组，后两者的基因组也存在显著差异，但绝对值相差较小。3种松毛虫雌雄虫的基因组大小之间也存在显著的差异，雌虫基因组大于雄虫。几种松毛虫基因组大小的测定，为进一步比较其分子差异、构建基因组文库和基因组学研究奠定了基础。 |
| Author | 张苏芳 张真 王鸿斌 孔祥波 罗基同 杨忠武 |
| AuthorAffiliation | 国家林业局森林保护学重点实验室,中国林业科学研究院森林生态环境与保护研究所,北京100091 广西壮族自治区森林病虫害防治站,广西南宁530022 广西壮族自治区桂林市森林病虫害防治检疫站,广西桂林541001 |
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| Author_FL | ZHANG Zhen ZHANG Su-fang WANG Hong-bin YANG Zhong-wu KONG Xiang-bo LUO Ji-tong |
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| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
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| DocumentTitleAlternate | Genome Size Determination of Several Dendrolimus Species in China |
| DocumentTitle_FL | Genome Size Determination of Several Dendrolimus Species in China |
| EndPage | 589 |
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| Keywords | 基因组大小 流式细胞仪 Dendrolimus chicken erythrocyte 鸡血细胞 flow cytometry genome size 松毛虫 |
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| Notes | ZHANG Su-fang, ZHANG Zhen, WANG Hong-bin , KONG Xiang-bo, LUO Ji-tong , YANG Zhong-wu （ 1. Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Beijing 100091, China; 2. Forest pest and disease control station of the Guangxi Zhuang Autonomous Region, Nanning 530022, Guangxi, China; 3. Forest pest and disease control station of Guilin city, the Guangxi Zhuang Autonomous Region, Guilin 541001 ） 11-1221/S flow cytometry, genome size, Dendrolimus, chicken erythrocyte Abstract： The genome size of three important Dendrolimus species in South China were measured by flow cytome- try, with chicken erythrocyte DNA content as control. The results showed that the genome size of D. punctatus was 563.36 ±7.26 Mb, and the 2C DNA content of female and male were 1. 1756±0.0164 and 1. 1286±0.0134 pg, respectively. The genome size of D. kikuchii was 719.30±9.70 Mb, and the 2C DNA content of female and male were 1. 4492±0. 0212 and 1. 4428±0. 0184 pg, respectively. The genome size of D. hou |
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| PublicationDate | 2014 |
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| PublicationTitle | 林业科学研究 |
| PublicationTitleAlternate | Forest Research |
| PublicationTitle_FL | Forest Research |
| PublicationYear | 2014 |
| Publisher | 国家林业局森林保护学重点实验室,中国林业科学研究院森林生态环境与保护研究所,北京100091%广西壮族自治区森林病虫害防治站,广西南宁,530022%广西壮族自治区桂林市森林病虫害防治检疫站,广西桂林,541001 |
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| Snippet | 本研究采用流式细胞术，以公鸡（Callus domesticus）血红细胞DNA含量为标准，测定了我国南方几种重要松毛虫的基因组大小（或称C-值）。结果显示，马尾松毛... S763; 本研究采用流式细胞术,以公鸡(Gallus domesticus)血红细胞DNA含量为标准,测定了我国南方几种重要松毛虫的基因组大小(或称C-值).结果显示,马尾松毛虫(Dendrolimus... |
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| SubjectTerms | 基因组大小 松毛虫 流式细胞仪 鸡血细胞 |
| Title | 我国几种重要松毛虫基因组大小测定 |
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