我国几种重要松毛虫基因组大小测定

本研究采用流式细胞术，以公鸡（Callus domesticus）血红细胞DNA含量为标准，测定了我国南方几种重要松毛虫的基因组大小（或称C-值）。结果显示，马尾松毛虫（Dendrolimuspunctatus）的基因组大小为563．36±7.26Mb，其雌、雄2C DNA含量分别为1．1756±0．0164和1．1286±0．0134Pg；思茅松毛虫（Dendrolimuskikuchii）的基因组大小为719．30±9．70Mb，其雌、雄2C DNA含量分别为1．4492±0．0212和1．4428±O．0184pg；云南松毛虫（Den-drolimushoui）的基因组大小为586．82...

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Published in林业科学研究 Vol. 27; no. 5; pp. 583 - 589
Main Author 张苏芳 张真 王鸿斌 孔祥波 罗基同 杨忠武
Format Journal Article
LanguageChinese
Published 国家林业局森林保护学重点实验室,中国林业科学研究院森林生态环境与保护研究所,北京100091%广西壮族自治区森林病虫害防治站,广西南宁,530022%广西壮族自治区桂林市森林病虫害防治检疫站,广西桂林,541001 2014
Subjects
基因组大小
松毛虫
流式细胞仪
鸡血细胞
基因组大小
流式细胞仪
Dendrolimus
chicken erythrocyte
鸡血细胞
flow cytometry
genome size
松毛虫
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ISSN1001-1498

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Summary:本研究采用流式细胞术，以公鸡（Callus domesticus）血红细胞DNA含量为标准，测定了我国南方几种重要松毛虫的基因组大小（或称C-值）。结果显示，马尾松毛虫（Dendrolimuspunctatus）的基因组大小为563．36±7.26Mb，其雌、雄2C DNA含量分别为1．1756±0．0164和1．1286±0．0134Pg；思茅松毛虫（Dendrolimuskikuchii）的基因组大小为719．30±9．70Mb，其雌、雄2C DNA含量分别为1．4492±0．0212和1．4428±O．0184pg；云南松毛虫（Den-drolimushoui）的基因组大小为586．82±8．94Mb，其雌、雄2CDNA含量分别为1．244±0．0182和1．156±O．0184Pg。统计分析结果发现，思茅松毛虫基因组显著大于云南松毛虫和马尾松毛虫基因组，后两者的基因组也存在显著差异，但绝对值相差较小。3种松毛虫雌雄虫的基因组大小之间也存在显著的差异，雌虫基因组大于雄虫。几种松毛虫基因组大小的测定，为进一步比较其分子差异、构建基因组文库和基因组学研究奠定了基础。
Bibliography:ZHANG Su-fang, ZHANG Zhen, WANG Hong-bin , KONG Xiang-bo, LUO Ji-tong , YANG Zhong-wu （ 1. Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Beijing 100091, China; 2. Forest pest and disease control station of the Guangxi Zhuang Autonomous Region, Nanning 530022, Guangxi, China; 3. Forest pest and disease control station of Guilin city, the Guangxi Zhuang Autonomous Region, Guilin 541001 ）
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flow cytometry, genome size, Dendrolimus, chicken erythrocyte
Abstract： The genome size of three important Dendrolimus species in South China were measured by flow cytome- try, with chicken erythrocyte DNA content as control. The results showed that the genome size of D. punctatus was 563.36 ±7.26 Mb, and the 2C DNA content of female and male were 1. 1756±0.0164 and 1. 1286±0.0134 pg, respectively. The genome size of D. kikuchii was 719.30±9.70 Mb, and the 2C DNA content of female and male were 1. 4492±0. 0212 and 1. 4428±0. 0184 pg, respectively. The genome size of D. hou
ISSN:1001-1498