广西主栽木薯品种体细胞胚的观察

以广西主栽木薯品种华南124（SC124）、新选048、辐选01以及非洲老品种TMS60444为试验材料,通过对SC124、新选048、辐选01和TMS60444的初级胚状体（华南124没有初级胚状体）、次级胚状体及TMS60444的胚性愈伤组织进行解剖学研究,采用石蜡切片法显微观察四种材料不同阶段胚状体的细胞微观形态。结果发现新选048、TMS60444和辐选01的初级胚状体细胞特征符合原分生组织特点,拥有非常旺盛的分裂能力,这可为鉴别胚性愈伤组织提供理论依据。...

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Bibliographic Details
Published in西南农业学报 Vol. 28; no. 6; pp. 2408 - 2412
Main Author 黎桦 黄贤教 林丽红 刘峰 钱梦丹 陈英之
Format Journal Article
LanguageChinese
Published 广西大学农学院,广西南宁,530005%广西大学生命科学与技术学院,广西南宁,530005 2015
Subjects
初级胚状体
木薯
次级胚状体
石蜡切片法
胚性愈伤组织
Manihot esculenta
胚性愈伤组织
初级胚状体
次级胚状体
Secondary somatic embryos
木薯
Primary somatic embryos
石蜡切片法
Paraffin-emhedded tissue
Embryogenic callus
Online AccessGet full text
ISSN1001-4829
DOI10.16213/j.cnki.scjas.2015.06.012

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Summary:以广西主栽木薯品种华南124（SC124）、新选048、辐选01以及非洲老品种TMS60444为试验材料,通过对SC124、新选048、辐选01和TMS60444的初级胚状体（华南124没有初级胚状体）、次级胚状体及TMS60444的胚性愈伤组织进行解剖学研究,采用石蜡切片法显微观察四种材料不同阶段胚状体的细胞微观形态。结果发现新选048、TMS60444和辐选01的初级胚状体细胞特征符合原分生组织特点,拥有非常旺盛的分裂能力,这可为鉴别胚性愈伤组织提供理论依据。
Bibliography:LI Hua;HUANG Xian-jiao;LIN Li-hong;LIU Feng;QIAN Meng-dan;CHEN Ying-zhi
51-1213/S
Primary somatic embryos; Secondary somatic embryos; Embryogenic callus; Paraffin-embedded tissue; Manihot esculenta
Four cassava cultivars viz.,SC124,Xinxuan 048 and Fuxuan 01（ main cultivar grown in Guangxi）,and Africa variety TMS60444 were taken as test materials to observe their embryoid cell micromorphology by anatomy research of primary embryoid,secondary embryoid or embryonic callus in present study. The paraffin section observations results showed that the characteristics of primary somatic cells of Xinxuan048,TMS60444 and Fuxuan 01 were accorded with the promeristem character,the cells had very vigours dividing ability,which would laid the theoretical basis for embryonic callus identification.
ISSN:1001-4829
DOI:10.16213/j.cnki.scjas.2015.06.012