火龙果两步成苗组培快繁技术研究

以火龙果茎段为材料,研究了不同外植体类型、激素配比和活性炭对火龙果组培快繁的影响。结果表明,保留小刺和绒毛的茎段更易于诱导不定芽,不定芽诱导的适宜培养基为MS＋6-BA 2～4 mg/L＋NAA 0.1 mg/L和MS＋TDZ 0.4～0.6 mg/L＋NAA0.1 mg/L,诱导率可达80%,火龙果不定芽增殖的优选培养基为MS＋6-BA 2 mg/L＋NAA 0.1 mg/L,增殖系数可达6.4,生根的优选培养基为MS＋NAA 0.3 mg/L＋活性炭1 g/L,生根率可达100%,茎段在MS＋6-BA 2～8 mg/L＋NAA 0.1 mg/L＋活性炭1mg/L培养基中增殖系数最高可达3....

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Bibliographic Details
Published in西南农业学报 Vol. 27; no. 6; pp. 2529 - 2533
Main Author 彭绿春 瞿素萍 苏艳 张艺萍 王丽花
Format Journal Article
LanguageChinese
Published 云南省花卉育种重点实验室,云南昆明650200 2014
国家观赏园艺工程技术中心,云南昆明650200
云南省农业科学院花卉研究所,云南昆明650200
云南花卉技术工程研究中心,云南昆明650200
国家观赏园艺工程技术中心,云南昆明650200%云南省农业科学院花卉研究所,云南昆明650200
农业部花卉产品质量监督检验测试中心(昆明),云南昆明650200
Subjects
两步成苗
快速繁殖
火龙果
组培
Hylocereus
undatus
两步成苗
two
steps
Rapid
快速繁殖
Grow
Tissue
propagation
culture
火龙果
plant
by
组培
to
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ISSN1001-4829
DOI10.16213/j.cnki.scjas.2014.06.051

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Summary:以火龙果茎段为材料,研究了不同外植体类型、激素配比和活性炭对火龙果组培快繁的影响。结果表明,保留小刺和绒毛的茎段更易于诱导不定芽,不定芽诱导的适宜培养基为MS＋6-BA 2～4 mg/L＋NAA 0.1 mg/L和MS＋TDZ 0.4～0.6 mg/L＋NAA0.1 mg/L,诱导率可达80%,火龙果不定芽增殖的优选培养基为MS＋6-BA 2 mg/L＋NAA 0.1 mg/L,增殖系数可达6.4,生根的优选培养基为MS＋NAA 0.3 mg/L＋活性炭1 g/L,生根率可达100%,茎段在MS＋6-BA 2～8 mg/L＋NAA 0.1 mg/L＋活性炭1mg/L培养基中增殖系数最高可达3.55,生根率为100%,实现了火龙果两步成苗快速繁殖。
Bibliography:51-1213/S
Hylocereus undatus; Grow to plant by two steps; Tissue culture; Rapid propagation
Hylocereus undatus stem was used as material to study the effect of explant type,hormone concentration and combination,active carbon（ ac） on rapid propagation. The result showed that the stem with small splinter and nap was easy to induce adventitious buds; the adapt medium were MS ＋ 6-BA 2- 4 mg / L ＋ NAA 0. 1 mg / L and MS ＋ TDZ 0. 2- 0. 4 mg / L ＋ NAA 0. 1 mg / L,the induction rate could reach 80 %,the best medium for adventitious buds propagation was MS ＋ 6-BA 2 mg / L ＋ NAA 0. 1 mg / L,the propagation coefficient could reach 6. 4,the best rooting medium was MS ＋ NAA 0. 3 mg / L ＋ ac 1 g / L,the rooting rate could reach 100 %,adventitious buds cultured on MS ＋ 6-BA 2- 8 mg / L ＋ NAA 0. 1 mg / L ＋ ac 1 g / L could grow to complete plant,the propagation coefficient could reach 3. 55,the rooting rate was 100 %.
PENG Lv-chun,QU Su-ping,SU Yan,ZHANG Yi-ping,WANG Li-hua（ 1. Flower Research Institute, Yunnan Academy of Agric
ISSN:1001-4829
DOI:10.16213/j.cnki.scjas.2014.06.051