耐冬山茶花粉储藏条件及其活力的动态变化

以山东青岛耐冬山茶花粉为试验材料,采用离体培养基法研究了花粉的萌发特性,并探讨了不同贮藏温度与贮藏时间对花粉萌发率和超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性的影响.结果表明:耐冬山茶花粉萌发的最适宜培养基为:150 g·L-1蔗糖+0.05 g·L-1硼酸+100 mg·L-1GA3+ 900 g·L-1Ca(NO3)2;花粉最佳贮藏温度为-80℃;-80℃贮藏360 d后,花粉萌发率仍达到原来的58.19%,3种保护酶活性较高,花粉抗氧化、衰老能力较强;贮藏过程中,3种保护酶活性出现最高峰值前后花粉萌发率出现大幅度下降;不同贮藏温度下,3种保护酶的作用不同,室...

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Published in林业科学研究 Vol. 28; no. 3; pp. 374 - 379
Main Author 贾文庆 王少平 范正琪 李纪元
Format Journal Article
LanguageChinese
Published 中国林业科学研究院亚热带林业研究所,浙江杭州311400 2015
河南科技学院园艺园林学院,河南新乡453003%河南科技学院园艺园林学院,河南新乡,453003%中国林业科学研究院亚热带林业研究所,浙江杭州,311400
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ISSN1001-1498

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Summary:以山东青岛耐冬山茶花粉为试验材料,采用离体培养基法研究了花粉的萌发特性,并探讨了不同贮藏温度与贮藏时间对花粉萌发率和超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性的影响.结果表明:耐冬山茶花粉萌发的最适宜培养基为:150 g·L-1蔗糖+0.05 g·L-1硼酸+100 mg·L-1GA3+ 900 g·L-1Ca(NO3)2;花粉最佳贮藏温度为-80℃;-80℃贮藏360 d后,花粉萌发率仍达到原来的58.19%,3种保护酶活性较高,花粉抗氧化、衰老能力较强;贮藏过程中,3种保护酶活性出现最高峰值前后花粉萌发率出现大幅度下降;不同贮藏温度下,3种保护酶的作用不同,室温下POD、CAT为敏感性保护酶,4℃下SOD为敏感性保护酶,-20℃、-80℃下CAT为敏感性保护酶;3种保护酶活性对花粉萌发率的影响依次为:SOD> POD> CAT.
Bibliography:11-1221/S
JIA Wen-qing, WANG Shao-ping , FAN Zheng-qi, LI Ji-yuan (1. Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou 311400, Zhejiang, China; 2. School of Horticulture Landscape Architecture, He' nan Institute of Science and Technology, Xinxiang 453003, He' nan, China)
Camellia japonica ; pollen; germination; storage; SOD; POD ; CAT
Fresh pollen of Camellia japonica was used to test the pollen viability by in vitro culture. The effects of different store methods on pollen viability and the activity of SOD, POD and CAT were also investigated. The results show that 1. The best combination for pollen germination was 150 g L-1 sucrose, 0.05 g L-1 boric acid, 100 mg L-1 GA3, and 900 g L-1 Ca(NO3) 2. 2. -80℃ sealed storage was the best storage condition to keep the vitality of the pollen. 3. After 360 days' storage, the activity of the three kinds of enzymes, the antioxidant capacity and aging ability of the pollen kept high. 4. The germination proportion of pollen decreased signific
ISSN:1001-1498