湖南省稻瘟病菌无毒基因鉴定

【目的】了解湖南省稻瘟病菌无毒基因组成,为培育水稻新品种及合理布局抗稻瘟病品种提供参考依据。【方法】采用2014年分离自湖南桃江病圃丽江新团黑谷（LTH）上的120个稻瘟病菌单孢菌株室内离体针刺接种于24个已知抗瘟基因的水稻近等基因系（NILs）上,对供试菌株进行无毒基因鉴定。【结果】120个稻瘟病菌含有全部24个无毒基因,在24个无毒基因中,出现频率高于40.00%的有Avr-Pita和Avr-Pish;出现频率在30.00%-40.00%的无毒基因有Avr-Piz、Avr-Piz5、Avr-Pi3和Avr-Pi9;其余无毒基因出现频率均较低,特别是Avr-Piks、Avr-Pikp、Avr...

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Bibliographic Details
Published in南方农业学报 Vol. 47; no. 9; pp. 1500 - 1505
Main Author 刘翔 任佐华 陈娟芳 毛锐 戴良英 刘二明
Format Journal Article
LanguageChinese
Published 湖南农业大学 植物保护学院/植物病虫害生物学与防控湖南省重点实室/南方粮油作物协同创新中心,长沙,410128 2016
Subjects
无毒基因
湖南省
离体接种
稻瘟病菌
近等基因系
稻瘟病菌
Hunan province
avirulent gene
无毒基因
near-isogenic lines(NILs)
湖南省
Magnaporthe oryzae
离体接种
in vitro inoculation
近等基因系
Online AccessGet full text
ISSN2095-1191
DOI10.3969/jissn.2095-1191.2016.09.1500

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Summary:【目的】了解湖南省稻瘟病菌无毒基因组成,为培育水稻新品种及合理布局抗稻瘟病品种提供参考依据。【方法】采用2014年分离自湖南桃江病圃丽江新团黑谷（LTH）上的120个稻瘟病菌单孢菌株室内离体针刺接种于24个已知抗瘟基因的水稻近等基因系（NILs）上,对供试菌株进行无毒基因鉴定。【结果】120个稻瘟病菌含有全部24个无毒基因,在24个无毒基因中,出现频率高于40.00%的有Avr-Pita和Avr-Pish;出现频率在30.00%-40.00%的无毒基因有Avr-Piz、Avr-Piz5、Avr-Pi3和Avr-Pi9;其余无毒基因出现频率均较低,特别是Avr-Piks、Avr-Pikp、Avr-Pib、Avr-Pi12和Avr-Pi11出现频率均低于10.00%。【结论】含抗性基因Pi-ta和Pi-sh的水稻品种可在湖南省推广利用;含抗性基因Pi-z、Pi-z5、Pi-3和Pi-9的水稻品种在湖南推广时应慎重考虑;含抗性基因Pi-ks、Pi-kp、Pi-b、Pi-12和Pi-11的水稻品种不宜在湖南省推广利用。
Bibliography:45-1381/S
[Objective]Composition of avirulent genes types of Magnaporthe oryzae in Hunan province were studied, in order to provide reference basis for breeding of new rice cultivars and rational distribution of blast -resistant cultivars.[Method]A total of 120 M. oryzae monadic isolates were isolated from rice susceptible cultivars （Lijiangxintuanheigu, LTH） in Hunan Taojiang nurseries in 2014. Then, 24 near-isogenic lines （NILs） with known blast-resistance gene was in vitro inoculated artificially with above isolates by acupuncture, and the avirulent genes of tested isolates were identified.[Result]The above 120 M. oryzae isolates all carried 24 avirulent genes, among 24 avirulent genes, occurrence frequencies of Avr-Pita and Avr-Pish were more than 40.00%, while occurrence frequencies of Avr-Piz, Avr-Piz5, Avr-Pi3 and Avr-Pi9 ranged from 30.00% to 40.00%, occurrence frequencies of the rest were lower relatively, especially Avr-Piks, Avr-Pikp, Avr-Pib, Avr-Pi12 and Avr-Pi11 with frequency of less than 10.00%
ISSN:2095-1191
DOI:10.3969/jissn.2095-1191.2016.09.1500