陆地棉低毒Bt基因对棉铃虫抗性的遗传效应分析

以Bt低毒表达的陆地棉种质系WG-20为试验材料,与若干Bt高毒表达的抗虫棉品系配制杂交组合,对其F1的Bt毒蛋白表达量、生物学抗虫性进行了分析。结果显示：Bt低毒表达性状对高毒表达性状表现为显性,生物学抗虫性也是低抗性状对抗级性状表现为显性,即低毒Bt基因对高毒Bt基因表现为显性抑制现象;Bt高毒表达材料之间杂交组合的F1则呈现Bt高毒表达,生物学抗性表达规律也是如此,即高毒Bt基因间不存在同源Bt基因表达的沉默现象。...

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Bibliographic Details
Published in江西农业学报 Vol. 28; no. 8; pp. 1 - 4
Main Author 陈旭升 狄佳春 赵亮
Format Journal Article
LanguageChinese
Published 江苏省农业科学院经济作物研究所/农业部长江下游棉花与油菜重点实验室,江苏南京,210014 2016
Subjects
低毒Bt基因
抗虫性
遗传效应
陆地棉
陆地棉
抗虫性
低毒Bt基因
Insect resistance
遗传效应
Genetic effect
Upland cotton
Low-toxic Bt gene
Online AccessGet full text
ISSN1001-8581
DOI10.19386/j.cnki.jxnyxb.2016.08.01

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Summary:以Bt低毒表达的陆地棉种质系WG-20为试验材料,与若干Bt高毒表达的抗虫棉品系配制杂交组合,对其F1的Bt毒蛋白表达量、生物学抗虫性进行了分析。结果显示：Bt低毒表达性状对高毒表达性状表现为显性,生物学抗虫性也是低抗性状对抗级性状表现为显性,即低毒Bt基因对高毒Bt基因表现为显性抑制现象;Bt高毒表达材料之间杂交组合的F1则呈现Bt高毒表达,生物学抗性表达规律也是如此,即高毒Bt基因间不存在同源Bt基因表达的沉默现象。
Bibliography:36-1124/S
Upland cotton;Low-toxic Bt gene;Insect resistance;Genetic effect
CHEN Xu-sheng,DI Jia-chun,ZHAO Liang（Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences / Key Laboratory of Cotton and Rape in Lower Rea- ches of Yangtze River, Ministry of Agriculture, Nanjing 210014, China）
In this study,the upland cotton germplasm WG- 20 with low- toxic Bt gene was used as experimental material.Several hybrid combinations were made by crossing between WG- 20 and some high-toxic-Bt insect-resistant cotton strains. The expression level of Bt toxalbumin,and the biological insect-resistance in F1 were analyzed. The results showed that the expression character of low-toxic Bt was dominant to that of high-toxic Bt,and the character of low insect-resistance was also dominant to that of high insect-resistance,namely the expression of low-toxic Bt gene dominantly inhibited to the expression of high-toxic Bt gene. The expression of high-toxic Bt gene was found in the F1 of hybrid combinations from high-toxic
ISSN:1001-8581
DOI:10.19386/j.cnki.jxnyxb.2016.08.01