催吐萝芙木内生细菌LYM3的分离鉴定及产抗菌活性物质的发酵条件优化

从催吐萝芙木中分离到1株对香蕉炭疽菌拮抗作用较强的菌株LYM3,皿内拮抗活性达到64%,通过常规鉴定结合16S r DNA序列分析,将该菌株鉴定为枯草芽孢杆菌（Bacillus subtilis）。通过单因素法对其产抗菌活性物质的发酵条件进行了优化,获得其最适培养基组成为：可溶性淀粉2%,胰蛋白胨1%,酵母粉0.8%,Na Cl 1%,初始p H 6.0、28℃、180 r/min发酵培养60 h,抗菌活性物质产量达到最高,抑菌圈直径为3.42cm。...

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Bibliographic Details
Published in广东农业科学 Vol. 42; no. 5; pp. 53 - 57
Main Author 李乔曼 吴亚腾 柳志强 李晓宇
Format Journal Article
LanguageChinese
Published 海南大学环境与植物保护学院,海南海口,570228 2015
Subjects
催吐萝芙木
内生细菌
分离鉴定
发酵条件优化
内生细菌
发酵条件优化
催吐萝芙木
分离鉴定
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ISSN1004-874X

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Summary:从催吐萝芙木中分离到1株对香蕉炭疽菌拮抗作用较强的菌株LYM3,皿内拮抗活性达到64%,通过常规鉴定结合16S r DNA序列分析,将该菌株鉴定为枯草芽孢杆菌（Bacillus subtilis）。通过单因素法对其产抗菌活性物质的发酵条件进行了优化,获得其最适培养基组成为：可溶性淀粉2%,胰蛋白胨1%,酵母粉0.8%,Na Cl 1%,初始p H 6.0、28℃、180 r/min发酵培养60 h,抗菌活性物质产量达到最高,抑菌圈直径为3.42cm。
Bibliography:44-1267/S
Rauvolfia vomitoria Afzel; endophytic bacteria; isolation and identification; optimization of fermentation condition
Strain LYM3, isolated from Rauvolfia vomitoria Afzel, had higher antagonistic effect against Colletotrichum musae, and its antagonistic activity was 64% in plate. According to its morphological characteristics,and 16 S r DNA sequence analysis, the strain LYM3 was identified as Bacillus subtilis. Using single-factor method,the fermentation conditions of its antibacterial substance production were optimized. The optimal medium contained2% soluble starch, 1% tryptone, 0.8% yeast extract, 1% Na Cl, and initial p H was 6. After 60 h culture at 28℃, 180r/min, the antibacterial substance had higher yield, and the diameter of inhibition zone was up to 3.42 cm
LI Qiao-man, WU Ya-teng, LIU Zhi-qiang, LI Xiao-yu （College of Environment and Plant Protection, Hainan University, Haikou 570228, China）
ISSN:1004-874X